Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
T Cell Types and Functions01:24

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Cells of the Adaptive Immune Response01:23

Cells of the Adaptive Immune Response

The T and B lymphocytes of the adaptive immune system develop from common lymphoid progenitor cells in the bone marrow. These progenitors give rise to precursors that eventually develop into both T and B lymphocytes. As these precursors mature, they gain the ability to detect and respond to foreign antigens in the body, a process known as immunocompetence. Additionally, these precursors acquire self-tolerance, a process that ensures they do not react to self-antigens. This intricate system...
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

Overview
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Nociceptor neurons suppress antitumor immunity in breast cancer.

Research square·2026
Same author

An autologous cell-based therapeutic vaccine expressing IL6/1 fusokine drives robust anti-tumor response against ovarian cancer.

bioRxiv : the preprint server for biology·2026
Same author

Noncanonical Transcription and Splicing Shape the Colorectal Cancer Immunopeptidome in MSI and MSS Tumors.

Molecular & cellular proteomics : MCP·2026
Same author

The oxidative phosphorylation inhibitor, atovaquone, upregulates PD-L1 via activation of the ATM/ATR DNA damage response pathway.

Research square·2026
Same author

Nociceptor neurons shape antiviral immunity.

Journal of neuroinflammation·2026
Same author

Extracellular vesicles mediate intercellular transfer of PD-L1 and bystander T-cell suppression.

Cellular and molecular life sciences : CMLS·2026

Related Experiment Video

Updated: May 30, 2026

Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor
10:33

Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor

Published on: December 7, 2015

Development and function of innate polyclonal TCRalphabeta+ CD8+ thymocytes.

Moutih Rafei1, Marie-Pierre Hardy, Patrick Williams

  • 1Institute for Research in Immunology and Cancer, University of Montreal, Montreal, Quebec H3C 3J7, Canada.

Journal of Immunology (Baltimore, Md. : 1950)
|August 17, 2011
PubMed
Summary
This summary is machine-generated.

Innate CD8 T cells are a significant population in normal thymuses, distinct from conventional T cells. These cells possess unique surface markers and gene expression, aiding conventional CD8 T cell expansion and cytokine secretion.

More Related Videos

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice
07:07

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

Published on: June 27, 2020

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
08:56

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Published on: June 9, 2015

Related Experiment Videos

Last Updated: May 30, 2026

Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor
10:33

Isolation and Ex Vivo Culture of Vδ1+CD4+γδ T Cells, an Extrathymic αβT-cell Progenitor

Published on: December 7, 2015

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice
07:07

Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice

Published on: June 27, 2020

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays
08:56

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

Published on: June 9, 2015

Area of Science:

  • Immunology
  • Cell Biology
  • Developmental Biology

Background:

  • The presence and origin of innate CD8 T cells in normal thymuses are debated.
  • Previous studies identified these cells primarily in mutant mouse models.

Purpose of the Study:

  • To investigate the existence and characteristics of innate CD8 T cells in normal thymuses.
  • To understand their development, function, and interactions with conventional T cells.

Main Methods:

  • Utilized the RAG2p-GFP mouse model for thymocyte analysis.
  • Characterized innate CD8 thymocytes by surface markers (e.g., CD44hi, GFP+) and gene expression (e.g., Eomes, T-bet).
  • Assessed positive selection, cytokine production (IFN-γ), proliferation, and helper functions.

Main Results:

  • Identified innate polyclonal T cells (GFP+CD44hi) comprising ~10% of TCRαβ(+) CD4(-)CD8(+) thymocytes.
  • Innate CD8 thymocytes show distinct surface markers (e.g., increased B7-H1, CD2, CD5) and transcript overexpression (e.g., Eomes).
  • These cells are positively selected by non-hematopoietic cells, produce high IFN-γ, proliferate with IL-2/IL-4, and help conventional CD8 T cells.

Conclusions:

  • Innate TCRαβ(+) CD8 T cells are a substantial subset of normal thymocytes.
  • Their development and function differ significantly from conventional CD8 T cells.
  • They play a role in supporting conventional CD8 T cell responses via CD44-mediated interactions.