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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: May 30, 2026

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Utilization of Plasmonic and Photonic Crystal Nanostructures for Enhanced Micro- and Nanoparticle Manipulation

Published on: September 27, 2011

Enhanced 3D fluorescence live cell imaging on nanoplasmonic substrate.

Manas Ranjan Gartia¹, Austin Hsiao, Mayandi Sivaguru

¹Department of Nuclear, Plasma and Radiological Engineering, University of Illinois, Urbana, IL 61801, USA.

|August 17, 2011

Summary

Researchers developed a silver-coated nanocone substrate for enhanced fluorescence detection. This novel substrate amplifies cellular fluorescence signals over 100-fold, enabling clearer 3D cell imaging.

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Published on: September 27, 2011

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Area of Science:

Nanotechnology
Biophotonics
Surface Plasmon Resonance

Background:

Surface plasmon resonance (SPR) is a powerful optical phenomenon utilized for sensitive detection.
Nanostructured substrates offer unique optical properties for enhancing light-matter interactions.
Efficient fluorescence detection is crucial for advanced biological imaging and diagnostics.

Purpose of the Study:

To develop a novel nanocone substrate for surface-plasmon-enhanced fluorescence detection.
To investigate the substrate's capability for three-dimensional (3D) cell imaging.
To quantify the fluorescence enhancement achieved on the nanocone substrate.

Main Methods:

Fabrication of a randomly distributed nanocone substrate on silicon, coated with silver.
Optical characterization to identify plasmonic modes within the 300-800 nm wavelength range.
Confocal fluorescence imaging of Chinese Hamster Ovary (CHO) cells cultured on the substrate.

Main Results:

The nanocone substrate supports multiple plasmonic modes that couple with fluorophores.
Plasmon resonance energy transfer (PRET) from the substrate to Rhodamine 6G (R6G) increases excitons and shortens fluorescence lifetime.
A >100-fold amplification in fluorescence intensity was observed for fluorophores on cell membranes compared to a glass substrate.
Three-dimensional fluorescence enhancement was demonstrated in CHO cells, with improved photostability.

Conclusions:

The silver-coated nanocone substrate effectively enhances fluorescence detection through surface plasmon resonance.
The substrate enables sensitive 3D cell imaging with significantly amplified fluorescence signals.
This technology holds promise for advanced biological imaging and diagnostic applications.