Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Matrix Metalloproteases in Degradation of ECM01:23

Role of Matrix Metalloproteases in Degradation of ECM

Matrix metalloproteases (MMPs) are enzymes involved in the hydrolysis of proteins and glycoproteins of the extracellular matrix. MMPs are essential for the migration and proliferation of cells through the dense matrix network, throughout embryonic development, and throughout morphogenesis. The first MMP activity discovered was a collagenase in a tadpole's tail undergoing metamorphosis. The active collagen deposition and modifications lead to the morphogenesis of tadpoles into the adult body.
A...
Antimicrobial Proteins01:23

Antimicrobial Proteins

Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
Determinants of Bacterial Pathogenicity and Virulence01:20

Determinants of Bacterial Pathogenicity and Virulence

Pathogenic bacteria employ a variety of strategies to establish infections, including the secretion of extracellular enzymes that act as potent virulence factors. These enzymes facilitate bacterial colonization of host tissues and help evade immune surveillance. By targeting structural components of host tissues and interfering with immune mechanisms, these enzymes play a pivotal role in disease progression.Extracellular Enzymes Facilitating Tissue Invasion: Several bacterial pathogens secrete...
Bacterial Toxins01:12

Bacterial Toxins

Bacterial toxins are sophisticated virulence factors that enable pathogenic bacteria to interact with, invade, and damage host tissues. These toxins fall broadly into two types: protein exotoxins, which are secreted into the environment and target specific host receptors, and lipopolysaccharide endotoxins, which are structural components of the bacterial outer membrane released primarily during bacterial lysis or membrane shedding. Exotoxins generally act more selectively, binding to cell...
Cytoskeletal Proteins in Bacteria01:29

Cytoskeletal Proteins in Bacteria

Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
Microbes and Other Elemental Cycles01:24

Microbes and Other Elemental Cycles

Microbial activity plays a pivotal role in the biogeochemical cycling of iron and manganese, especially at the redox gradients characteristic of stratified aquatic environments. These cycles are driven by microbial transformations between oxidized and reduced forms of the metals, allowing organisms to exploit them for metabolic energy and structural purposes.Iron Cycling Across Redox GradientsIn neutral, oxygen-rich surface waters, iron is predominantly found in its oxidized, insoluble ferric...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

D-Amino Acids in Marine and Terrestrial Environments: Diversity and Uniformity of Their Microbial Catabolism.

Environmental microbiology·2026
Same author

Multimodular flavobacterial enzymes specialized in coordinated decomposition of cellulose and alginate in brown algal cell walls.

The ISME journal·2026
Same author

Structural insights into bacterial dimethylsulfoniopropionate import by BCCT-family transporters.

The EMBO journal·2026
Same author

Diversity analysis of phytase-producing bacteria from coastal seawater and sediment and characterization of their phytases.

Engineering microbiology·2026
Same author

Structure and energy transfer of a far-red-absorbing euglenophyte PSI-LhcE-LhcbM supercomplex.

Nature communications·2026
Same author

Metabolic Regulation of Dimethylsulfoniopropionate Cleavage and Dimethyl Sulfide Production in Halomonas sp. D47.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Related Experiment Video

Updated: May 30, 2026

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography
07:32

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography

Published on: March 30, 2022

Extracellular metalloproteases from bacteria.

Ji-Wei Wu1, Xiu-Lan Chen

  • 1Dezhou University, China.

Applied Microbiology and Biotechnology
|August 17, 2011
PubMed
Summary

Bacterial extracellular metalloproteases (BEMPs) are diverse enzymes crucial for bacterial nutrition and widely used in industry. Further research and engineering can expand their applications.

Area of Science:

  • Microbiology
  • Biochemistry
  • Enzymology

Background:

  • Bacterial extracellular metalloproteases (BEMPs) are secreted by heterotrophic bacteria.
  • They are metal-containing enzymes with diverse structural and functional characteristics.

Purpose of the Study:

  • To review the diversity, structure, maturation, roles, and applications of BEMPs.
  • To highlight the potential for novel BEMP discovery and engineering.

Main Methods:

  • Literature review of BEMP research.
  • Analysis of BEMP classification, structure, and function.
  • Discussion of industrial applications and future directions.

Main Results:

  • BEMPs are classified into nine families based on sequence and structure.

More Related Videos

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases
07:20

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases

Published on: August 1, 2025

Expression, Purification, and Antimicrobial Activity of S100A12
11:10

Expression, Purification, and Antimicrobial Activity of S100A12

Published on: May 13, 2017

Related Experiment Videos

Last Updated: May 30, 2026

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography
07:32

Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography

Published on: March 30, 2022

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases
07:20

One-step Extraction and Zymographic Analysis of Bacterial Gelatinases

Published on: August 1, 2025

Expression, Purification, and Antimicrobial Activity of S100A12
11:10

Expression, Purification, and Antimicrobial Activity of S100A12

Published on: May 13, 2017

  • They function as endoproteases, typically with a catalytic Zn(2+).
  • Maturation involves propeptide removal via proteolysis or autocleavage.

    • Conclusions:

    • BEMPs are vital for bacterial nutrient acquisition through environmental protein degradation.
    • Their varied enzymology aids adaptation to diverse environments.
    • Current applications in food and pharmaceuticals can be expanded through novel discovery and protein engineering.