Deciphering the regulatory codes in bacterial genomes
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Updated: May 30, 2026

DNA-affinity-purified Chip (DAP-chip) Method to Determine Gene Targets for Bacterial Two component Regulatory Systems
Published on: July 21, 2014
Byung-Kwan Cho1, Bernhard Palsson, Karsten Zengler
1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, Korea. bcho@kaist.ac.kr
Abstract:
Interactions between cis-regulatory elements and trans-acting factors are fundamental for cellular functions such as transcription. With the revolution in microarrays and sequencing technologies, genome-wide binding locations of trans-acting factors are being determined in large numbers. The richness of the genome-scale information has revealed that the nature of the bacterial transcriptome and regulome are considerably more complex than previously expected. In addition, the emerging view of the bacterial transcriptome is revising the concept of the operon organization of the genome. This review describes current advances in the genome-scale analysis of the interaction between cis-regulatory elements and trans-acting factors in microorganisms.
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