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Updated: May 30, 2026
Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
[Establishment of NCAM L1 minigene model and its splicing patterns in different cell lines]
Wei Zhang1, Quan Shen, Zheng-yu Peng
1School of Medicine and Life Science, Zhejiang University City College, Hangzhou 310015, China.
Objective:
To establish a minigene model of neural cell adhesion molecule L1 (NCAM L1) gene and to study its splicing patterns in different cell lines.
Methods:
Using human genetic cDNA as template, the NCAM L1 minigene fragment was amplified and inserted into eukaryotic expression vector. The minigene was transfected into 4 cell lines and the splicing patterns of NCAM L1 minigene in these cell lines were studied.
Results:
The splicing patterns of NCAM L1 minigene were different in individual cell lines. In PFSK and Hela cell lines, two splicied isoforms were generated but in COS-1 and R28 cell lines, only one isoform existed.
Conclusion:
NCAM L1 minigene model can be used in alternative splicing analysis.
