Orosensory stimulation effects on human saliva proteome
Katharina Lorenz1, Matthias Bader, Andreas Klaus
1Food Chemistry and Molecular Sensory Science, Technische Universität München, Lise-Meitner Strasse 34, D-85354 Freising, Germany.
Journal of Agricultural and Food Chemistry
|August 18, 2011
Summary
Oral stimulation with 6-gingerol, sanshools, and citric acid alters saliva proteome. Specific proteins like SPLUNC2 and Zn-α-GP increase, potentially enhancing mucosal defense mechanisms.
Area of Science:
- Biochemistry
- Proteomics
- Immunology
Background:
- Saliva composition dynamically changes in response to various stimuli.
- Understanding saliva proteome modulation is crucial for comprehending oral health and defense mechanisms.
Purpose of the Study:
- To investigate the time-dependent changes in the whole saliva proteome induced by different oral stimuli.
- To identify specific salivary proteins affected by 6-gingerol, hydroxy-α/β-sanshools, and citric acid.
Main Methods:
- Saliva flow was induced by 6-gingerol, sanshools, and citric acid.
- Whole saliva proteome analysis using 2D-PAGE, tryptic in-gel digestion, and MALDI-TOF-MS peptide mass fingerprinting.
Main Results:
- 6-Gingerol stimulation decreased proteins including glutathione S-transferase P and annexin A1.
- Increased abundance of prolactin inducible proteins (PIP), SPLUNC2, Zn-α-GP, and CAVI was observed.
- Proteome modulation likely resulted from protein release from preformed vesicles, not de novo synthesis.
Conclusions:
- Elevated SPLUNC2, Zn-α-GP, and CAVI may activate innate mucosal immunity and non-immune defense.
- These proteins could play a significant role in the early stages of oral inflammation.
- Salivary proteomic analysis provides insights into rapid oral protective responses.
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