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Updated: May 30, 2026
High-Resolution Complexome Profiling by Cryoslicing BN-MS Analysis
Published on: October 15, 2019
Factor Xa subsite mapping by proteome-derived peptide libraries improved using WebPICS, a resource for proteomic
Oliver Schilling1, Ulrich auf dem Keller, Christopher M Overall
1Institute for Molecular Medicine and Cell Research, University of Freiburg, Germany. oliver.schilling@mol-med.uni-freiburg.de
Abstract:
Proteomic identification of protease cleavage site specificity (PICS) is a recent proteomic approach for the easy mapping of protease subsite preferences that determines both the prime- and non-prime side specificity concurrently. Here we greatly facilitate user access by providing an automated and simple web-based data-analysis resource termed WebPics (http://clipserve.clip.ubc.ca/pics/). We demonstrate the utility of WebPics analysis of PICS data by determining the substrate specificity of factor Xa from P6-P6', an important blood coagulation protease that proteolytically generates thrombin from prothrombin. PICS confirms existing data on non-prime site specificity and refines our knowledge of factor Xa prime-site selectivity.
