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Updated: May 30, 2026

Differentiation of the SH-SY5Y Human Neuroblastoma Cell Line
Published on: February 17, 2016
Ago1 and Ago2 differentially affect cell proliferation, motility and apoptosis when overexpressed in SH-SY5Y
Chiara Parisi1, Corinna Giorgi, Enrico Maria Batassa
1Department of Cellular Biotechnology and Hematology, Sapienza University, Rome, Italy.
Abstract:
Argonaute are a conserved class of proteins central to the microRNA pathway. We have highlighted a novel and non-redundant function of Ago1 versus Ago2; the two core factors of the miRNA-associated RISC complex. Stable overexpression of Ago1 in neuroblastoma cells causes the cell cycle to slow down, a decrease in cellular motility and a stronger apoptotic response upon UV irradiation. These effects, together with a significant increase in p53 levels, suggest that Ago1 may act as a tumor-suppressor factor, a function also supported by GEO Profiles microarrays that inversely correlate Ago1 expression levels with cell proliferation rates.
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