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Updated: May 30, 2026
05:39
Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice
Published on: July 8, 2020
NF-kB expression in IgA nephropathy outcome
G E B Silva1, R S Costa, R C Ravinal
1Department of Pathology, University of São Paulo, Ribeirão, Preto, Brazil.
Disease Markers
|August 18, 2011
Summary
Increased nuclear factor-kappa B (NF-kB) expression in kidney tubules predicts a poor prognosis for IgA nephropathy (IgAN) patients. Southwestern histochemistry (SWH) better correlates NF-kB levels with adverse outcomes than immunohistochemistry (IHC).
Area of Science:
- Nephrology
- Immunopathology
- Molecular Biology
Background:
- Glomerulonephritis pathogenesis involves nuclear factor-kappa B (NF-kB).
- Primary IgA nephropathy (IgAN) is a common form of glomerulonephritis with variable prognosis.
Purpose of the Study:
- To evaluate the prognostic significance of NF-kB expression in IgAN.
- To compare NF-kB detection methods: immunohistochemistry (IHC) and southwestern histochemistry (SWH).
Main Methods:
- Analysis of 62 IgAN patients diagnosed between 1987 and 2003.
- Utilized antibodies for CD68, mast cell tryptase, TGF-β1, α-SMA, and NF-kB p65.
- Employed SWH for in situ detection of activated NF-kB.
Main Results:
- NF-kB expression, particularly by SWH, correlated with clinical and histological parameters.
- Tubular NF-kB expression by SWH predicted an unfavorable IgAN clinical course.
- Increased NF-kB expression (IHC and SWH) was linked to reduced renal survival.
Conclusions:
- Elevated tubular NF-kB expression may indicate poor prognosis in IgAN.
- SWH demonstrated a stronger correlation with adverse outcomes compared to IHC for NF-kB detection.
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