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Related Experiment Video

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Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice
05:39

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Published on: July 8, 2020

NF-kB expression in IgA nephropathy outcome.

G E B Silva1, R S Costa, R C Ravinal

  • 1Department of Pathology, University of São Paulo, Ribeirão, Preto, Brazil.

Disease Markers
|August 18, 2011
PubMed
Summary
This summary is machine-generated.

Increased nuclear factor-kappa B (NF-kB) expression in kidney tubules predicts a poor prognosis for IgA nephropathy (IgAN) patients. Southwestern histochemistry (SWH) better correlates NF-kB levels with adverse outcomes than immunohistochemistry (IHC).

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Dioscin Mediated IgA Nephropathy Alleviation by Inhibiting B Cell Activation In Vivo and Decreasing Galactose-Deficient IgA1 Production In Vitro
14:18

Dioscin Mediated IgA Nephropathy Alleviation by Inhibiting B Cell Activation In Vivo and Decreasing Galactose-Deficient IgA1 Production In Vitro

Published on: October 13, 2023

Area of Science:

  • Nephrology
  • Immunopathology
  • Molecular Biology

Background:

  • Glomerulonephritis pathogenesis involves nuclear factor-kappa B (NF-kB).
  • Primary IgA nephropathy (IgAN) is a common form of glomerulonephritis with variable prognosis.

Purpose of the Study:

  • To evaluate the prognostic significance of NF-kB expression in IgAN.
  • To compare NF-kB detection methods: immunohistochemistry (IHC) and southwestern histochemistry (SWH).

Main Methods:

  • Analysis of 62 IgAN patients diagnosed between 1987 and 2003.
  • Utilized antibodies for CD68, mast cell tryptase, TGF-β1, α-SMA, and NF-kB p65.
  • Employed SWH for in situ detection of activated NF-kB.

Main Results:

  • NF-kB expression, particularly by SWH, correlated with clinical and histological parameters.
  • Tubular NF-kB expression by SWH predicted an unfavorable IgAN clinical course.
  • Increased NF-kB expression (IHC and SWH) was linked to reduced renal survival.

Conclusions:

  • Elevated tubular NF-kB expression may indicate poor prognosis in IgAN.
  • SWH demonstrated a stronger correlation with adverse outcomes compared to IHC for NF-kB detection.