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Related Experiment Video

Updated: May 30, 2026

Super-Resolution Live Cell Imaging of Subcellular Structures

Super-Resolution Live Cell Imaging of Subcellular Structures

Published on: January 13, 2021

Full-field supercritical angle fluorescence microscopy for live cell imaging.

Thomas Barroca¹, Karla Balaa, Julie Delahaye

¹Centre d'Imageries Plasmoniques Appliquées, Institut Langevin, Ecole Supérieure de Physique et de Chimie Industrielles (ESPCI) ParisTech, CNRS UMR 7587, Université Paris Diderot, 10 rue Vauquelin, 75 231 Paris Cedex 05, France.

|August 18, 2011

Summary

This study presents a new fluorescence imaging method for precise 100 nm axial confinement at interfaces. The technique enables real-time observation of live cell surface and internal dynamics.

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Area of Science:

Biophysics
Cell Biology
Optical Imaging

Background:

Standard surface imaging techniques lack precise axial confinement.
Observing live cell membrane and adhesion dynamics requires advanced imaging methods.

Purpose of the Study:

Introduce a novel full-field fluorescence imaging technique.
Achieve axial confinement of approximately 100 nm at the sample/substrate interface.
Enable real-time surface imaging for live cell studies.

Main Methods:

Utilize emission filtering based on supercritical emission selectivity.
Implement the technique on standard epifluorescence microscopes with high numerical aperture objectives.
Apply the method to human embryonic kidney cells.

Main Results:

Demonstrate axial confinement of ~100 nm at the sample interface.
Achieve real-time surface imaging capabilities.
Successfully observe simultaneous surface and in-depth phenomena in live cells.

Conclusions:

The developed technique provides precise axial confinement for advanced surface imaging.
This method is suitable for studying live cell membrane and adhesion dynamics.
It offers a versatile tool for observing both surface and internal cellular processes concurrently.