Related Experiment Video

Updated: May 30, 2026

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
11:13

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Published on: May 9, 2019

Characteristics of low-density and high-density lipoprotein subclasses in pediatric renal transplant recipients

Aleksandra Zeljkovic1, Jelena Vekic, Vesna Spasojevic-Kalimanovska

  • 1Department of Medical Biochemistry, Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia. aleksandra.zeljkovic@pharmacy.bg.ac.rs

Transplant International : Official Journal of the European Society for Organ Transplantation
|August 19, 2011
PubMed

Insights

Pediatric renal transplant recipients exhibit altered high-density lipoprotein (HDL) and low-density lipoprotein (LDL) particle profiles, linked to cardiovascular risk. Specific HDL subclasses and small, dense LDL (sdLDL) levels correlate with carotid intima-media thickness (cIMT).

Area of Science:

  • Lipidology
  • Cardiovascular Research
  • Pediatric Nephrology

Background:

  • Dyslipidemia is a major cardiovascular disease risk factor in renal transplant recipients.
  • Understanding lipoprotein particle characteristics is crucial for managing cardiovascular risk in this population.

Purpose of the Study:

  • To characterize high-density lipoprotein (HDL) and low-density lipoprotein (LDL) particles in pediatric renal transplant recipients.
  • To investigate the association between lipoprotein particle characteristics and carotid intima-media thickness (cIMT).
  • To examine the impact of immunosuppressive therapy on lipoprotein profiles.

Main Methods:

  • Gradient gel electrophoresis for HDL size and subclass distribution.
  • Heparin-magnesium precipitation for small, dense LDL (sdLDL)-cholesterol (sdLDL-C) and sdLDL-apolipoprotein B (sdLDL-apoB) quantification.
  • Comparison between 21 pediatric renal transplant recipients and 32 controls.

Main Results:

  • Renal transplant recipients showed reduced HDL 2b but increased HDL 3a and 3b subclasses.
  • Elevated sdLDL-C and sdLDL-apoB levels were observed in recipients.
  • The proportion of HDL 3b subclasses predicted increased cIMT.
  • Cyclosporine treatment was associated with higher sdLDL-C and sdLDL-apoB compared to tacrolimus.

Conclusions:

  • Pediatric renal transplant recipients have abnormal HDL and LDL particle distribution.
  • Altered small HDL particle proportions are linked to increased cIMT.
  • Advanced lipid testing can aid in assessing immunosuppressive therapy effects on lipid profiles.

More Related Videos

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation06:47

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Published on: January 28, 2021

Related Concept Videos

Pharmacokinetics in Pediatric Patients: Drug Distribution01:17

Pharmacokinetics in Pediatric Patients: Drug Distribution

Drug distribution in the pediatric population exhibits unique challenges and considerations due to the physiological differences between children, particularly neonates and infants, and adults. A crucial aspect of pediatric pharmacology is understanding how these differences impact the pharmacokinetics of various drugs, necessitating age-specific dosing strategies to ensure efficacy and safety.Neonates and infants have a higher total body water content, ~75%–90% of their body weight, compared...
Pharmacokinetics in Pediatric Patients: Drug Excretion01:26

Pharmacokinetics in Pediatric Patients: Drug Excretion

In pediatric medicine, understanding the renal function and drug elimination nuances is crucial for administering safe and effective treatments. Newborns, in particular, display markedly slower renal functions than adults, profoundly affecting how drugs are cleared from their bodies. This slower drug clearance requires clinicians to extend the dosing intervals for many medications to prevent drug accumulation and toxicity while ensuring therapeutic efficacy.One key area where these adjustments...
Kidney Transplant I: Introduction01:28

Kidney Transplant I: Introduction

A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
Pharmacokinetics in Pediatric Patients: Drug Metabolism01:24

Pharmacokinetics in Pediatric Patients: Drug Metabolism

In pediatric care, understanding the nuances of hepatic drug metabolism is crucial, as it significantly differs from that of adults. This divergence is primarily due to the developmental stage of drug-metabolizing enzymes, which affects how medications are processed in the body. In neonates, for instance, the activity of Phase I enzymes—critical for the initial breakdown of drugs—is markedly reduced, functioning at just 20–40% of the levels seen in adults. This reduction poses a challenge in...
Kidney Transplant III: Nursing Management01:16

Kidney Transplant III: Nursing Management

Postoperative Nursing Management for Kidney Transplant PatientsPostoperative nursing management care includes monitoring the surgical site, encouraging early movement, and promoting lung health through breathing exercises. Nurses also administer prescribed medications like H2-blockers, such as famotidine, or proton pump inhibitors, like omeprazole, to help prevent gastrointestinal ulcers and bleeding. Fungal infections in the mouth and bladder can result from immunosuppressive and antibiotic...
Pharmacokinetics in Pediatric Patients: Overview and Drug Absorption01:23

Pharmacokinetics in Pediatric Patients: Overview and Drug Absorption

Understanding the physiological differences in the pediatric population is crucial for effective pharmacotherapy. Neonates, infants, and children exhibit significant variations in gastric pH, gastric emptying time, intestinal transit time, and biliary function. These variations profoundly affect oral drug absorption, necessitating a nuanced approach to pediatric dosing.Neonates present with a unique physiological profile, having a gastric pH greater than 4 and faster and more irregular gastric...