Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 30, 2026

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering
07:09

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Published on: April 30, 2017

Engineering of vascularized adipose constructs.

Paul S Wiggenhauser1, Daniel F Müller, Ferry P W Melchels

  • 1Department for Plastic and Hand Surgery, Klinikum Rechts der Isar, Technische Universität München, Ismaninger Straße 22, Munich, Germany.

Cell and Tissue Research
|August 19, 2011
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scaffold-free tenogenic macro-tissues engineered by multi-layered bioassembly of rat tendon fibroblast spheroids.

Biofabrication·2026
Same author

Quantifying Morphological Change in Stage III Lipedema: A 3D Imaging Study of Population Trends and Individual Treatment Courses.

Journal of personalized medicine·2025
Same author

[Free Microvascular Lymph Node Transplantation from the Omentum for the Treatment of Lymphoedema].

Zentralblatt fur Chirurgie·2025
Same author

Elastic osmotic capsules mimic bolus injection with high release at delayed burst.

International journal of pharmaceutics·2025
Same author

Gellan gum-based granular gels as suspension media for biofabrication.

PloS one·2024
Same author

Investigating the Thickness of the Deltoid Free Flap Using Ultrasonography and Clinical Application in Foot and Hand Soft-Tissue Defect Reconstruction.

Journal of reconstructive microsurgery·2024

This study explored polyurethane and polycaprolactone scaffolds for adipose tissue engineering. Both scaffolds supported cell growth and new blood vessel formation in vivo, with polyurethane showing slightly better results for fibrous tissue formation and adipogenesis.

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Adipose tissue engineering is a promising alternative for soft tissue defect repair.
  • Developing suitable scaffolds for customized tissue constructs, especially for breast surgery, remains a challenge.

Purpose of the Study:

  • To investigate the potential of two distinct scaffold types for adipose tissue regeneration.
  • To compare polyurethane foam and polycaprolactone scaffolds in supporting cell growth, differentiation, and in vivo tissue formation.

Main Methods:

  • Fabrication of sponge-like polyurethane scaffolds via mold casting.
  • Fabrication of polycaprolactone scaffolds with stacked-fiber architecture using fused deposition modeling.
  • Seeding scaffolds with human adipose-derived precursor cells, in vitro culture, and in vivo implantation in nude mice with a flow-through model for angiogenesis.

More Related Videos

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
09:55

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Published on: May 30, 2016

Engineered Vascularized Muscle Flap
08:18

Engineered Vascularized Muscle Flap

Published on: January 11, 2016

Related Experiment Videos

Last Updated: May 30, 2026

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering
07:09

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

Published on: April 30, 2017

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber
09:55

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Published on: May 30, 2016

Engineered Vascularized Muscle Flap
08:18

Engineered Vascularized Muscle Flap

Published on: January 11, 2016

Main Results:

  • Human adipose-derived precursor cells attached to and differentiated into adipocytes on both scaffold types in vitro.
  • In vivo, both scaffolds promoted angiogenesis and adipose tissue formation within 2 and 4 weeks.
  • Polyurethane foam scaffolds exhibited more pronounced fibrous tissue formation and adipogenesis compared to polycaprolactone scaffolds.

Conclusions:

  • Both polyurethane and polycaprolactone scaffolds are effective for adipose tissue engineering.
  • Polyurethane foam scaffolds demonstrated superior performance in promoting fibrous tissue formation and adipogenesis in this study.