Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complement System01:27

Complement System

The complement system is a group of approximately 20 plasma proteins that strengthen the body's defenses against infections through opsonization, inflammation, and cell lysis. Opsonization involves coating pathogens with complement proteins, making them more recognizable and facilitating phagocyte engulfment. Certain complement proteins induce inflammation that attracts immune cells to the site of infection. Cell lysis involves the destruction of pathogens through the formation of a membrane...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Protein Complex Assembly02:41

Protein Complex Assembly

Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Assembly of Signaling Complexes01:30

Assembly of Signaling Complexes

Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
Interaction domains in cell signaling
Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin, triggering...
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Characterization of response-adapted surgery following neoadjuvant systemic therapy in oral cavity cancer.

Oral oncology·2026
Same author

Reconsidering Surgical Strategy in the Era of Neoadjuvant Immunotherapy for Head and Neck Cancer.

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery·2026
Same author

SpyGlass-Assisted Percutaneous Transhepatic Cholangioscopy for Benign and Malignant Biliary Strictures: A Retrospective Observational Study.

Journal of medical imaging and radiation oncology·2026
Same author

Longitudinal <i>in vivo</i> human wound healing model defines key role for smooth muscle cells in ECM remodeling.

bioRxiv : the preprint server for biology·2026
Same author

SpyGlass-Assisted Percutaneous Transhepatic Cholangioscopy in Patients With Biliary Duct Calculi: A Retrospective Cohort Study From an Australasian Centre.

Journal of medical imaging and radiation oncology·2026
Same author

Expanding the Histologic Spectrum of Human Papillomavirus (HPV)-Associated Oropharyngeal Carcinoma: A Retrospective Study of 379 Cases Focusing on Classification, Histologic Features, and their Prognostic Significance.

Head and neck pathology·2026

Related Experiment Video

Updated: May 30, 2026

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

Membrane attack by complement: the assembly and biology of terminal complement complexes.

Cosmin A Tegla1, Cornelia Cudrici, Snehal Patel

  • 1Department of Neurology, School of Medicine, University of Maryland, 655 W. Baltimore Street, BRB 12-033, Baltimore, MD 21201, USA.

Immunologic Research
|August 19, 2011
PubMed
Summary
This summary is machine-generated.

Sublytic complement C5b-9 channels promote cell cycle progression and inhibit apoptosis in nucleated cells. This process is crucial for cell activation, proliferation, differentiation, and maintaining tissue homeostasis.

More Related Videos

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
06:54

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Published on: June 15, 2019

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)
06:45

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)

Published on: June 15, 2018

Related Experiment Videos

Last Updated: May 30, 2026

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment
07:26

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion
06:54

Evaluation of the Interplay Between the Complement Protein C1q and Hyaluronic Acid in Promoting Cell Adhesion

Published on: June 15, 2019

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)
06:45

Dissecting Multi-protein Signaling Complexes by Bimolecular Complementation Affinity Purification (BiCAP)

Published on: June 15, 2018

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Complement system activation is vital for immunity.
  • The membrane attack complex (C5b-9) typically causes cell death.
  • Sublytic C5b-9, however, can modulate cellular processes.

Purpose of the Study:

  • To investigate the role of sublytic C5b-9 in cell cycle progression.
  • To elucidate the molecular mechanisms by which C5b-9 influences cell fate.
  • To understand C5b-9's impact on apoptosis and cell proliferation.

Main Methods:

  • Analysis of signal transduction pathways (PI3K/Akt/FOXO1, ERK1).
  • Assessment of cell cycle regulators (CDK4, CDK2, RGC-32).
  • Investigation of apoptosis-related molecules (Bad, FLIP, caspase-8, Bid).

Main Results:

  • Sublytic C5b-9 induces cell cycle progression via PI3K/Akt/FOXO1 and ERK1 pathways.
  • C5b-9 activates CDK4, CDK2, and RGC-32, promoting G1/S transition and proliferation.
  • C5b-9 inhibits apoptosis by phosphorylating Bad and blocking key apoptotic factors.

Conclusions:

  • Sublytic C5b-9 promotes cell activation, proliferation, and differentiation.
  • These effects contribute to the maintenance of cell and tissue homeostasis.
  • C5b-9 acts as a signaling molecule influencing cell fate beyond cell lysis.