Related Experiment Videos
NMR studies of a bacterial cell culture medium (LB broth): cyclic nucleotides in yeast extracts
M H Rayner1, P J Sadler, M D Scawen
1Department of Chemistry, Birkbeck College, Gordon House, Laboratory, London, U.K.
FEMS Microbiology Letters
|March 1, 1990
Abstract:
The composition of LB broth (tryptone, yeast extract and NaCl) was investigated by 1H,31P-NMR spectroscopy, FPLC and gel electrophoresis. An unexpected finding was the high level of 2'3'-cyclic nucleotides, detected by characteristic 31P-NMR resonances in the region 20-21 ppm, originating from the yeast component. 31P-NMR resonances for cyclic nucleotides were observed during the autolysis of Saccharomyces cerevisiae cells, and in model reactions of RNase with RNA.