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Characterization of the defect in the Escherichia coli mutT1 mutator gene
S K Bhatnagar1, L C Bullions, G Lew
1McCollum-Pratt Institute, Johns Hopkins University, Baltimore, Maryland 21218.
Journal of Bacteriology
|May 1, 1990
Abstract:
With a probe constructed from the wild-type gene, a DNA fragment containing the entire mutT1 mutator gene was isolated and cloned into pUC18. Nucleotide sequence analysis revealed that the mutator defect was most likely due to an IS1 insertion into the wild-type gene.