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Human seminal clusterin (SP-40,40). Isolation and characterization.

M K O'Bryan1, H W Baker, J R Saunders

  • 1St. Vincent's Hospital, Melbourne, Australia.

The Journal of Clinical Investigation
|May 1, 1990
PubMed
Summary

Human seminal clusterin, also known as sulfated glycoprotein-2, is purified and characterized. Its higher concentration in seminal plasma suggests a key role in male fertility.

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Area of Science:

  • Reproductive Biology
  • Immunology
  • Biochemistry

Background:

  • SP-40,40, a human complement inhibitor, shows homology to rat and ram Sertoli cell product, sulfated glycoprotein-2 (clusterin).
  • Clusterin is found in various body fluids, including serum.

Purpose of the Study:

  • To purify and characterize human seminal clusterin.
  • To investigate the concentration and potential role of seminal clusterin in male fertility.

Main Methods:

  • Purification and characterization of human seminal clusterin.
  • Two-dimensional gel electrophoresis to compare seminal and serum clusterin.
  • Hemagglutination and hemolysis inhibition assays.
  • Measurement of clusterin concentration in seminal plasma and serum.

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  • Immunofluorescence of human spermatozoa.
  • Pilot study correlating seminal clusterin with in vitro fertilization rates.

    • Main Results:

    • Human seminal clusterin was purified and characterized, showing charge differences from serum-derived clusterin.
    • Both seminal and serum clusterin exhibited hemagglutination and complement inhibition activity.
    • Seminal plasma clusterin concentrations were significantly higher than in serum.
    • Lower seminal clusterin levels were observed in azoospermia and oligospermia compared to normospermia.
    • Undetectable seminal clusterin in vasal agenesis suggests seminal vesicle production.
    • Clusterin was detected on immature and morphologically abnormal spermatozoa.
    • Seminal clusterin concentration correlated with in vitro fertilization rates in a pilot study.

    Conclusions:

    • Human seminal clusterin is biochemically distinct from serum clusterin but shares functional activities.
    • Seminal clusterin concentration is significantly higher in seminal plasma than serum, with reduced levels in certain infertility conditions.
    • Evidence suggests seminal vesicles contribute to seminal clusterin production.
    • The presence of clusterin on spermatozoa and its correlation with fertilization rates indicate a potential role in male reproductive function.