Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Coagulase testing compared with commercial kits for routinely identifying Staphylococcus aureus.

A S Rossney1, L F English, C T Keane

  • 1Microbiology Department, St James's Hospital, Dublin, Ireland.

Journal of Clinical Pathology
|March 1, 1990
PubMed
Summary

Commercial Staphylococcus aureus identification kits were evaluated against traditional coagulase testing methods. Results show that these kits do not outperform established tube coagulase tests for routine laboratory identification of S. aureus.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Venous catheter associated candida albicans septicaemia.

Irish journal of medical science·2016
Same author

Transmission of endemic ST22-MRSA-IV on four acute hospital wards investigated using a combination of spa, dru and pulsed-field gel electrophoresis typing.

European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology·2012
Same author

The Diagnostic Value of Leukocyte Stains in Leukaemias.

Postgraduate medical journal·2011
Same author

Isolation rates of meticillin-resistant Staphylococcus aureus in dogs, cats and horses in Ireland.

The Veterinary record·2010
Same author

When are the hands of healthcare workers positive for methicillin-resistant Staphylococcus aureus?

The Journal of hospital infection·2010
Same author

Spread of community-acquired meticillin-resistant Staphylococcus aureus skin and soft-tissue infection within a family: implications for antibiotic therapy and prevention.

Journal of medical microbiology·2010

Area of Science:

  • Clinical Microbiology
  • Bacteriology

Background:

  • Accurate identification of Staphylococcus aureus is crucial in clinical settings.
  • Commercial kits offer potential for rapid identification, but their routine efficacy requires validation.

Purpose of the Study:

  • To evaluate the performance of five commercial Staphylococcus aureus identification kits.
  • To compare kit performance against tube coagulase and slide coagulase testing methods.
  • To assess the impact of selective media and storage conditions on identification accuracy.

Main Methods:

  • Five commercial kits (Staphaurex, Staphylase, Staphyslide, Biostaph, Bacto Latex) were tested.
  • Comparison included two tube coagulase methods and five slide coagulase methods.
  • Organisms tested included staphylococcal and mixed bacterial species groups.

Related Experiment Videos

  • Effects of selective media and isolate storage were investigated.

    • Main Results:

    • Selective media and prolonged isolate storage were not recommended.
    • Tube coagulase reference method achieved 100% efficiency.
    • Commercial kits ranged from 87% to 94% efficiency.
    • Best performing kits showed minimal advantage over slide coagulase tests.

    Conclusions:

    • Commercial Staphylococcus aureus identification kits should not replace tube coagulase testing in routine laboratories.
    • Even high-performing kits offer limited advantages over established slide coagulase methods.
    • Tube coagulase testing remains the gold standard for S. aureus identification from primary plates.