Related Experiment Video
Updated: May 30, 2026
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
Published on: October 15, 2013
High-content screening in microfluidic devices
Raymond Cheong1, Saurabh Paliwal, Andre Levchenko
1Johns Hopkins University, Department of Biomedical Engineering, 3400 N. Charles St., Baltimore, MD 21218, USA.
Importance Of The Field:
Miniaturization is the key to advancing the state of the art in high-content screening (HCS) in order to enable dramatic cost savings through reduced usage of expensive biochemical reagents and to enable large-scale screening on primary cells. Microfluidic technology offers the potential to enable HCS to be performed with an unprecedented degree of miniaturization.
Areas Covered In This Review:
This perspective highlights a real-world example from the authors’ work of HCS assays implemented in a highly miniaturized microfluidic format. The advantages of this technology are discussed, including cost savings, high-throughput screening on primary cells, improved accuracy, the ability to study complex time-varying stimuli, and ease of automation, integration and scaling.
What The Reader Will Gain:
The reader will understand the capabilities of anew microfluidics-based platform for HCS and the advantages it provides over conventional plate-based HCS.
Take Home Message:
Microfluidics technology will drive significant advancements and broader usage and applicability of HCS in drug discovery.
