Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon has three reading...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Qingfei Paidu decoction, a compound Chinese herbal medicine, protects against post-influenza bacterial infection by inhibiting the formation of neutrophil extracellular traps and cytokine storm.

Journal of integrative medicine·2026
Same author

Longitudinal <sup>1</sup>H and <sup>129</sup>Xe Lung MRI in Patients With Post-COVID Residual Lung Abnormalities.

Journal of magnetic resonance imaging : JMRI·2026
Same author

HLA-E-restricted T cells primed by a modified HLA-B*57:01 restricted HIV-1 peptide suppress HIV-1 replication.

JCI insight·2026
Same author

Nonclassical MHC-I Molecules: Emerging Therapeutic Targets in Next-Generation Immunotherapy.

MedComm·2026
Same author

Investigating prognostic classifications of preexisting multiple long-term conditions for health outcomes 1 year after COVID-19 hospitalization: A UK prospective observational study.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026
Same author

Consortium-Based Patient and Public Involvement and Engagement for Long COVID Research: A Pirit-Focused Impact Evaluation of the PHOSP-COVID Study.

Health expectations : an international journal of public participation in health care and health policy·2026
Same journal

Epidemiological characteristics of amebiasis in Japan from 2001 to 2022.

PloS one·2026
Same journal

Longitudinal associations of academic stress with eating related patterns, nutrition, somatic indicators, and depressive symptoms in university students: A study protocol.

PloS one·2026
Same journal

Pollution removal efficiency enhancement by agricultural biomass additions in constructed wetlands: A framework integrating meta-analysis with explainable machine learning.

PloS one·2026
Same journal

Insulation failure mapping on power transformer bushing using FRA and electrostatic simulation.

PloS one·2026
Same journal

Enhancing medical Q&A systems with multimodal knowledge graphs and dual-layer attention mechanisms.

PloS one·2026
Same journal

UAMP: Consistent video object segmentation with uncertainty-aware memory propagation.

PloS one·2026
See all related articles

Related Experiment Video

Updated: May 30, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Alternative spliced CD1d transcripts in human bronchial epithelial cells.

Kambez Hajipouran Benam1, Wai Ling Kok, Andrew J McMichael

  • 1MRC Human Immunology Unit, Weatherall Institute of Molecular Medicine, University of Oxford, Oxford, United Kingdom.

Plos One
|August 20, 2011
PubMed
Summary
This summary is machine-generated.

Researchers discovered CD1d molecule variants and protein expression in human bronchial epithelial cells, suggesting a new role for CD1d in lung mucosal immunity and host defense.

More Related Videos

Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
08:42

Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures

Published on: May 26, 2023

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Related Experiment Videos

Last Updated: May 30, 2026

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition
11:48

Detection of Alternative Splicing During Epithelial-Mesenchymal Transition

Published on: October 9, 2014

Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures
08:42

Isolating Bronchial Epithelial Cells from Resected Lung Tissue for Biobanking and Establishing Well-Differentiated Air-Liquid Interface Cultures

Published on: May 26, 2023

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

Area of Science:

  • Immunology
  • Molecular Biology
  • Respiratory Medicine

Background:

  • CD1d is an MHC Class I-like molecule presenting glycolipids to natural killer T (NKT) cells.
  • NKT cells are crucial for immune regulation against infections and autoimmune diseases.
  • CD1d expression is known on antigen-presenting cells and some non-hematopoietic cells, but not previously on bronchial epithelium.

Purpose of the Study:

  • To investigate the presence and characteristics of CD1d in human bronchial epithelial cells.
  • To identify potential alternatively spliced CD1D mRNA variants in this cell type.
  • To explore the implications of CD1d expression in lung mucosal immunity.

Main Methods:

  • RT-PCR to identify CD1D mRNA variants.
  • Western blotting or immunohistochemistry to detect CD1d protein expression.
  • Analysis of alternative splicing patterns in bronchial epithelial cells.

Main Results:

  • Identification of CD1D mRNA variants in human bronchial epithelial cells.
  • Detection of CD1d protein expression on these cells.
  • Characterization of six alternatively spliced CD1D transcripts specific to epithelial cells.

Conclusions:

  • Human bronchial epithelial cells express CD1D mRNA variants and CD1d protein.
  • These findings suggest a novel role for CD1d in lung mucosal immunity.
  • This discovery opens avenues for studying CD1d's function in respiratory host defense.