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Updated: May 30, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Effect of dilution on high-density lipoprotein associated paraoxonase-1 activity
Mike Mackness1, Bharti Mackness
1PONTECH ART, Calle de L'Argelagar, 43840 Salou, Tarragona, Spain. mike.mackness@gmail.com
Objectives:
To investigate the effects of dilution on high-density lipoprotein (HDL) associated paraoxonase-1 (PON1) activity.
Design And Methods:
HDL was prepared by ultracentrifugation, diluted with TRIS buffer pH 8.2 (0-128 fold) and assayed spectrophotometrically for PON1 activity using paraoxon and phenyl acetate.
Results:
HDL-PON1 activity increased progressively with dilution to over 200% of the undiluted activity by 128 fold dilution (P<0.001). Conversely HDL size decreased.
Conclusion:
These results indicate the urgent need to standardise conditions for measuring PON1 activity.
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