Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is the future of cardiac repair cell-free?

European heart journal·2026
Same author

A causal 'lnc' between exercise and ageing in the cardiac vasculature.

European heart journal·2026
Same author

The dark genome in cardiovascular medicine.

European heart journal·2026
Same author

Adiponectin exerts sex-dependent effects on lipid, amino acid, and glucose metabolism during caloric restriction.

PLoS biology·2026
Same author

Vein graft failure: Pathophysiology, detection, prevention and emerging therapeutic strategies.

Pharmacological reviews·2026
Same author

Myocardial Fibroblast Activation in Ischemic and Nonischemic Cardiomyopathy.

JAMA cardiology·2026

Related Experiment Video

Updated: May 30, 2026

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

MicroRNAs regulating cell pluripotency and vascular differentiation.

Lynsey Howard1, Nicole M Kane, Graeme Milligan

  • 1BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical, Veterinary and Life Sciences, University of Glasgow, 126 University Place, Glasgow G12 8TA, UK.

Vascular Pharmacology
|August 23, 2011
PubMed
Summary

Human embryonic stem cells (hESC) can generate vascular cells for regenerative medicine. MicroRNAs (miRNAs) are key regulators controlling hESC pluripotency and cardiovascular differentiation for tissue repair.

More Related Videos

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
11:51

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Published on: May 11, 2020

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Related Experiment Videos

Last Updated: May 30, 2026

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs
14:41

RNA Blot Analysis for the Detection and Quantification of Plant MicroRNAs

Published on: July 11, 2020

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.
11:51

Potato Virus X-Based microRNA Silencing (VbMS) In Potato.

Published on: May 11, 2020

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
10:48

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

Published on: April 12, 2015

Area of Science:

  • Stem cell biology
  • Regenerative medicine
  • Molecular biology

Background:

  • Human embryonic stem cells (hESC) possess self-renewal and differentiation capabilities, making them promising for regenerative medicine.
  • hESC are a potential source for vascular cells to repair ischemic tissues.
  • Precise control over hESC pluripotency and differentiation is crucial for therapeutic applications.

Purpose of the Study:

  • To review the role of microRNAs (miRNAs) in maintaining hESC pluripotency.
  • To examine miRNA involvement in differentiating hESC into cardiovascular lineages (endothelial cells, vascular smooth muscle cells, cardiomyocytes).
  • To contextualize miRNA functions for cardiovascular regenerative medicine.

Main Methods:

  • Literature review of studies on miRNAs, hESC, and cardiovascular differentiation.
  • Analysis of miRNA regulatory mechanisms in stem cell maintenance and lineage commitment.
  • Synthesis of findings related to regenerative medicine strategies.

Main Results:

  • MicroRNAs are identified as critical negative regulators of gene expression.
  • Specific miRNAs are implicated in both sustaining pluripotency and directing cardiovascular differentiation of hESC.
  • Understanding these miRNA roles is essential for controlled cell generation.

Conclusions:

  • MicroRNAs are pivotal in regulating hESC fate decisions.
  • Targeting miRNAs offers a strategy for generating specific cardiovascular cell types from hESC for regenerative therapies.
  • Further research into miRNA-mediated differentiation holds significant potential for cardiovascular medicine.