Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Heterochromatin02:38

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at 9th...
Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Euchromatin01:01

Euchromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions take up more dye, appearing darker, while the less-compact areas take up less dye and appear lighter. Based on the compaction level, chromatins are classified into two primary forms – euchromatin and heterochromatin.
Euchromatin is the less dense region of the chromatin and stains lighter. Euchromatin contains histone H3 extensively...
Oogenesis01:22

Oogenesis

Oogenesis,  the process of developing egg cells (female gametes), occurs within the ovaries and is fundamental to female fertility. This sequence begins during fetal development when diploid oogonia in the developing ovaries undergo mitotic divisions to produce primary oocytes. By birth, these primary oocytes enter prophase I of meiosis but become arrested in this stage, remaining suspended until puberty.
Each primary oocyte is surrounded by a layer of pre-granulosa cells, forming what is known...
Oogenesis02:07

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Use of mariner transposases for one-step delivery and integration of DNA in prokaryotes and eukaryotes by transfection.

Nucleic acids research·2017
Same author

Structural Basis for the Inverted Repeat Preferences of mariner Transposases.

The Journal of biological chemistry·2015
Same author

Biochemical characterization and comparison of two closely related active mariner transposases.

Biochemistry·2014
Same author

Retrotransposons.

Current biology : CB·2012
Same author

RNA:RNA interaction can enhance RNA localization in Drosophila oocytes.

RNA (New York, N.Y.)·2012
Same author

Molecular architecture of the Mos1 paired-end complex: the structural basis of DNA transposition in a eukaryote.

Cell·2009
Same journal

Hunting ecology predicts eye arrangements in the modular visual system of spiders.

Current biology : CB·2026
Same journal

Sub-second fluctuations between top-down and bottom-up modes distinguish diverse human brain states.

Current biology : CB·2026
Same journal

Queen bees offload pesticide burden to eggs when social buffering is overwhelmed.

Current biology : CB·2026
Same journal

Pitch selectivity in ferret auditory cortex.

Current biology : CB·2026
Same journal

A cell size-dependent competition between geometry and polarity governs nuclear and spindle positioning in early embryos.

Current biology : CB·2026
Same journal

Trophic cascades drive sustainability in the agricultural heritage rice-fish coculture system.

Current biology : CB·2026
See all related articles

Related Experiment Video

Updated: May 30, 2026

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
10:39

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

Published on: February 26, 2018

Oogenesis: active heterochromatin.

David J Finnegan1

  • 1Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, King's Buildings, Mayfield Road, Edinburgh EH9 3JR, UK. david.finnegan@ed.ac.uk

Current Biology : CB
|August 23, 2011
PubMed
Summary
This summary is machine-generated.

Short RNAs protect the Drosophila genome from DNA damage during oogenesis. Surprisingly, their transcription requires DNA associated with gene-silencing histone modifications, revealing a novel regulatory mechanism.

More Related Videos

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization
07:16

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

Published on: January 7, 2016

Detection of DNA Double-Stranded Breaks in Mouse Oocytes
07:46

Detection of DNA Double-Stranded Breaks in Mouse Oocytes

Published on: June 23, 2023

Related Experiment Videos

Last Updated: May 30, 2026

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II
10:39

Chromatin Spread Preparations for the Analysis of Mouse Oocyte Progression from Prophase to Metaphase II

Published on: February 26, 2018

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization
07:16

Isolation and Characterization of Mouse Antral Oocytes Based on Nucleolar Chromatin Organization

Published on: January 7, 2016

Detection of DNA Double-Stranded Breaks in Mouse Oocytes
07:46

Detection of DNA Double-Stranded Breaks in Mouse Oocytes

Published on: June 23, 2023

Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • The genome requires protection from DNA damage, particularly during sensitive developmental processes like oogenesis.
  • Short RNAs are known to play roles in various cellular processes, including genome regulation.

Purpose of the Study:

  • To investigate the mechanism by which the Drosophila genome is protected from DNA damage during oogenesis.
  • To elucidate the role of short RNAs and associated regulatory elements in this protective process.

Main Methods:

  • Analysis of gene expression patterns during Drosophila oogenesis.
  • Investigation of DNA-associated histone modifications.
  • Characterization of short RNA populations and their biogenesis.

Main Results:

  • A mechanism involving short RNAs was identified that protects the Drosophila genome from DNA damage during oogenesis.
  • Transcription of these protective short RNAs unexpectedly requires their DNA to be associated with a histone modification typically linked to gene silencing.

Conclusions:

  • Short RNAs play a crucial role in safeguarding the genome during Drosophila oogenesis.
  • A novel regulatory pathway is revealed where gene-silencing marks facilitate the transcription of protective small RNAs, highlighting a paradoxical mechanism for genome defense.