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Published on: February 14, 2016
Bovine fetal DNA in the maternal circulation: Applications and implications
D C Lemos1, P L Takeuchi, A F L Rios
1Departamento de Genética, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil. lemosdc@gmail.com
Objective:
The main aim of the present study was to detect bovine fetal DNA in the maternal circulation, a relatively unexplored subject in the literature.
Study Design:
DNA was extracted from blood of 84 primipara cows (Bos indicus) at different gestational ages (30-270 days) and from 100 adult animals (50 males and 50 non-pregnant cows). The samples were analyzed using PCR with primers for TSPY gene.
Results:
Molecular results matched the fetal phenotypic gender in all 47 male and 37 female fetuses, including early pregnancy, and in control animals.
Conclusions:
These results evidence a bovine transplacental fetal DNA passage.
