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Updated: May 30, 2026

Assay for Adhesion and Agar Invasion in S. cerevisiae

Assay for Adhesion and Agar Invasion in S. cerevisiae

Published on: November 8, 2006

Functional artificial free-standing yeast biofilms.

Svetlana A Konnova¹, Mehmet Kahraman, Alsu I Zamaleeva

¹Biomaterials and Nanomaterials Group, Department of Biochemistry, Kazan (Idel buye) Federal University, Kreml uramı 18, Kazan, Republic of Tatarstan 420008, Russian Federation.

Colloids and Surfaces. B, Biointerfaces

|August 23, 2011

Summary

Researchers created artificial free-standing biofilms using layer-by-layer assembly and sacrificial templates. These biofilms mimic primitive multicellular organisms and can incorporate various microparticles for added functionality.

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Last Updated: May 30, 2026

Assay for Adhesion and Agar Invasion in S. cerevisiae

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Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

Pseudomonas aeruginosa and Saccharomyces cerevisiae Biofilm in Flow Cells

Published on: January 15, 2011

Area of Science:

Biomaterials Science
Microbiology
Synthetic Biology

Background:

Biofilms are complex microbial communities crucial in various environments.
Current methods for biofilm formation often require specific substrates.
Mimicking natural biofilms with artificial constructs offers new research avenues.

Purpose of the Study:

To develop a novel method for fabricating free-standing artificial biofilms.
To incorporate live cells and functional microparticles into artificial biofilms.
To create versatile artificial biofilms for potential applications in synthetic biology and cell culture.

Main Methods:

Utilized sacrificial calcium carbonate-coated templates for biofilm construction.
Employed layer-by-layer assembly of polyelectrolytes and live yeast cells.
Incorporated magnetic, latex, and silver micro- and nanoparticles into the biofilm structure.
Developed symbiotic biofilms containing both yeast and bacteria.

Main Results:

Successfully fabricated free-standing, multilayered artificial biofilms.
Demonstrated the incorporation of live yeast cells and various micro/nanoparticles.
Showcased the creation of functional biofilms with magnetic, latex, and silver components.
Developed symbiotic multicellular biofilms integrating yeast and bacteria.

Conclusions:

The developed method enables the fabrication of substrate-free artificial biofilms.
These artificial biofilms mimic primitive multicellular and colonial species.
The approach offers a versatile platform for creating functional artificial biofilms and symbiotic microbial consortia.
This technique can advance the development of artificial colonial microorganisms and cell culture systems.