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Published on: August 23, 2024
Enterococcus plantarum sp. nov., isolated from plants.
Pavel Švec1, Peter Vandamme2, Hana Bryndová1
1Czech Collection of Microorganisms, Department of Experimental Biology, Faculty of Science, Masaryk University, Tvrdého 14, 602 00 Brno, Czech Republic.
Researchers discovered a new bacterial species, Enterococcus plantarum, isolated from plants. This novel species is distinct from known enterococci, confirmed by genetic and phenotypic analyses.
Area of Science:
- Microbiology
- Bacteriology
- Plant Science
Background:
- Enterococci are common bacteria found in various environments.
- Screening plant-associated microbial communities can reveal novel bacterial species.
Purpose of the Study:
- To characterize novel bacterial isolates from plant surfaces.
- To determine the taxonomic position of these isolates within the genus Enterococcus.
Main Methods:
- rep-PCR fingerprinting using the (GTG)(5) primer.
- 16S rRNA gene sequencing and phylogenetic analysis.
- Sequencing of pheS and rpoA genes.
- Whole-cell protein fingerprinting, automated ribotyping, and extensive phenotyping.
Main Results:
- Eight Gram-positive, catalase-negative bacterial strains were isolated.
- The isolates formed a distinct cluster separate from known Enterococcus species.
- Phylogenetic analyses confirmed the isolates belong to the genus Enterococcus but represent a novel species.
- Genetic and phenotypic homogeneity was observed among the isolates.
Conclusions:
- The isolates represent a novel species, Enterococcus plantarum sp. nov.
- The type strain is CCM 7889(T).
- This discovery expands the known diversity of Enterococcus species associated with plants.
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