Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Resolving the mechanical paradox of myelination.

Communications biology·2026
Same author

Metabolic profiling of steatotic liver disease by fluorescence lifetime imaging microscopy.

Communications medicine·2026
Same author

Actin waves guide an outward movement of microclusters in the lymphocyte immunological synapse.

EMBO reports·2025
Same author

Protocol for lattice light-sheet time-lapse imaging of early post-implantation mouse embryos.

STAR protocols·2025
Same author

Digital holographic microscopy for rapid bacteria segmentation and counting in microfluidic cartridges: basic considerations and limitations for diagnostic application.

Journal of biomedical optics·2025
Same author

Electroretinography With the RM Electrode: Normative Reference Ranges, Variation With Age, and Comparison With the Burian-Allen Electrode.

Translational vision science & technology·2025
Same journal

Multifunctional reconfigurable terahertz metasurface based on vanadium dioxide phase transition: achieving broadband absorption and efficient polarization conversion.

Applied optics·2026
Same journal

High-Q-factor electromagnetically induced transparency utilizing quasi-bound states in the continuum in an all-dielectric terahertz metasurface.

Applied optics·2026
Same journal

Automated stitching interferometry for high-precision metrology of X-ray mirrors.

Applied optics·2026
Same journal

Experimental demonstration of an approach to designing a metal-dielectric DBR resonant cavity structure.

Applied optics·2026
Same journal

High-precision wavefront reconstruction from a single-shot interferogram using a physics-driven hybrid feature calibration network.

Applied optics·2026
Same journal

Ultra-high-Q Fano resonance based on coupled topological corner states in Kagome photonic crystals.

Applied optics·2026
See all related articles

Related Experiment Video

Updated: May 30, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

High-resolution digital holography utilized by the subpixel sampling method.

Daniel Claus1, Marco Fritzsche, Daciana Iliescu

  • 1Kroto Research Institute, University of Sheffield, S3 7HQ Sheffield, UK. d.claus@sheffield.ac.uk

Applied Optics
|August 23, 2011
PubMed
Summary
This summary is machine-generated.

This study introduces a new digital holography method using a complementary metal-oxide semiconductor (CMOS) camera for subpixel resolution. This approach enhances optical resolution, image quality, and phase measurement accuracy in holographic recordings.

More Related Videos

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
07:27

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Published on: November 1, 2017

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Related Experiment Videos

Last Updated: May 30, 2026

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
10:28

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)
07:27

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Published on: November 1, 2017

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
10:16

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Area of Science:

  • Optics and Photonics
  • Digital Imaging
  • Holography

Background:

  • Digital holography techniques often face limitations in achievable resolution.
  • Improving image quality and phase accuracy is crucial for advanced holographic applications.

Purpose of the Study:

  • To present a novel method for enhancing resolution in digital holography.
  • To leverage complementary metal-oxide semiconductor (CMOS) sensor capabilities for improved holographic data acquisition.

Main Methods:

  • Recording interference fields using a CMOS camera with subpixel resolution capabilities.
  • Developing experimental and numerical procedures to implement the subpixel recording technique.
  • Addressing challenges related to small pixel size and reduced fill factor in CMOS sensors.

Main Results:

  • Demonstrated significant improvement in optical resolution.
  • Showcased enhanced image quality in reconstructed holograms.
  • Validated increased accuracy in phase measurements.

Conclusions:

  • The proposed subpixel resolution approach offers a viable solution for resolution enhancement in digital holography.
  • This method effectively improves key performance metrics including resolution, image quality, and phase accuracy.
  • The technique holds potential for broader applications in scientific and industrial imaging.