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Published on: September 21, 2011
GPR50 interacts with TIP60 to modulate glucocorticoid receptor signalling
Jian Li1, Laura E Hand, Qing-Jun Meng
1Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom.
Plos One
|August 23, 2011
Summary
G protein-coupled receptor 50 (GPR50) interacts with TIP60, modulating glucocorticoid receptor signaling. This discovery clarifies GPR50
Area of Science:
- Endocrinology
- Molecular Biology
- Cell Biology
Background:
- G protein-coupled receptor 50 (GPR50) is an orphan receptor related to melatonin receptors.
- The precise physiological role of GPR50, particularly in energy homeostasis, requires further elucidation.
Purpose of the Study:
- To investigate the functional interactions of GPR50 with other cellular proteins.
- To determine GPR50's role in modulating transcriptional co-activator activity and downstream signaling pathways.
Main Methods:
- Yeast-two-hybrid screening to identify interacting proteins.
- Co-immunoprecipitation and co-localization assays to confirm protein interactions.
- In vivo studies using Gpr50 knockout mice to assess physiological relevance.
Main Results:
- GPR50 was identified as a signaling partner of the transcriptional co-activator TIP60.
- Co-expression of GPR50 and TIP60 influenced the subcellular localization of GPR50.
- GPR50 enhances TIP60-mediated coactivation of glucocorticoid receptor (GR) signaling.
- GR-mediated effects on Pomc expression and gluconeogenesis were altered in Gpr50(-/-) mice.
Conclusions:
- GPR50 plays a novel role in regulating glucocorticoid receptor signaling via its interaction with TIP60.
- This interaction impacts key physiological processes regulated by the glucocorticoid receptor.
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