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Related Concept Videos

RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Nucleic Acid Structure01:25

Nucleic Acid Structure

The pentose sugar in DNA is deoxyribose, while in RNA the pentose sugar is ribose. The difference between the sugars is the presence of the hydroxyl group on the ribose's second carbon and a hydrogen on the deoxyribose's second carbon. The phosphate residue attaches to the hydroxyl group of the 5′ carbon of one sugar and the hydroxyl group of the 3′ carbon of the sugar of the next nucleotide, which forms  a 5′ to 3′ phosphodiester linkage.
DNA Structure
DNA has a double-helix structure. The...
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
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Matrin 3 binds and stabilizes mRNA.

Maayan Salton1, Ran Elkon, Tatiana Borodina

  • 1The David and Inez Myers Laboratory for Cancer Research, Department of Human Molecular Genetics and Biochemistry, Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel. maayan.salton@gmail.com

Plos One
|August 23, 2011
PubMed
Summary
This summary is machine-generated.

Matrin 3 (MATR3) protein binds RNA and influences gene expression. This study reveals MATR3

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Biotin-based Pulldown Assay to Validate mRNA Targets of Cellular miRNAs
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Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Nuclear Matrix Proteins

Background:

  • Matrin 3 (MATR3) is an inner nuclear matrix protein with poorly understood functions.
  • MATR3 is phosphorylated by ATM kinase, linking it to DNA damage response.
  • The protein contains zinc finger domains and RNA recognition motifs (RRM).

Purpose of the Study:

  • To investigate the function of Matrin 3 (MATR3) in RNA processing and gene regulation.
  • To identify RNA species and protein interactions associated with MATR3.
  • To determine MATR3's role in mRNA stability and transcription.

Main Methods:

  • RNA immunoprecipitation followed by deep sequencing (RIP-seq) to identify bound RNAs.
  • Microarray analysis to assess transcript levels in the presence and absence of MATR3.
  • Validation of transcript binding to the MATR3 complex.

Main Results:

  • MATR3 interacts with RNA in an RNA-dependent manner via its RRM2 domain.
  • RIP-seq identified small noncoding RNAs bound to MATR3.
  • Microarray analysis revealed 77 transcripts whose levels depend on MATR3, with nine validated.
  • MATR3 was shown to be crucial for the stability of several mRNA species.

Conclusions:

  • MATR3 plays a significant role in mRNA stabilization.
  • The cellular levels of MATR3 modulate the stability of specific gene transcripts.
  • MATR3 is involved in RNA processing and gene expression regulation.