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Related Experiment Video

Updated: May 30, 2026

Dynamic Light Scattering Analysis for the Determination of the Particle Size of Iron-Carbohydrate Complexes
04:40

Dynamic Light Scattering Analysis for the Determination of the Particle Size of Iron-Carbohydrate Complexes

Published on: July 7, 2023

Dynamic light scattering as an efficient tool to study glyconanoparticle-lectin interactions.

Xin Wang1, Olof Ramström, Mingdi Yan

  • 1Department of Chemistry, Portland State University, P.O. Box 751, Portland, Oregon 97207-0751, USA.

The Analyst
|August 23, 2011
PubMed
Summary

Dynamic light scattering efficiently studies glyconanoparticle-lectin interactions. This method enables sensitive detection and high-affinity binding measurements for various nanomaterials.

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Last Updated: May 30, 2026

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Glyconanomaterials (GNMs) are emerging bio-functional nanomaterials with applications in detecting, imaging, and targeting biological entities like proteins and cells.
  • Studying the interactions between GNMs and lectins is crucial for developing advanced biosensing and diagnostic tools.

Purpose of the Study:

  • To establish dynamic light scattering (DLS) as an efficient method for investigating glyconanoparticle (GNP)--lectin interactions.
  • To quantify the sensitivity and binding affinity of GNP-lectin interactions using DLS.

Main Methods:

  • Silica and gold nanoparticles (NPs) were conjugated with D-mannose (Man) or D-galactose (Gal).
  • These GNPs were treated with lectins Concanavalin A (Con A) and Ricinus communis agglutinin (RCA(120)).
  • Hydrodynamic volumes of the resulting aggregates were measured using DLS to assess particle size changes.

Main Results:

  • Particle size increased with rising lectin concentration, indicating GNP-lectin complex formation.
  • The limit of detection (LOD) was as low as 2.9 nM for Con A with Man-conjugated silica NPs.
  • DLS measurements revealed 3-4 orders of magnitude higher binding affinity of GNPs compared to free ligands.

Conclusions:

  • DLS is a versatile and efficient tool for studying GNP-lectin interactions.
  • The sensitivity and affinity of the assay are dependent on nanoparticle size.
  • This DLS-based method is general and applicable to various nanomaterial compositions for biosensing applications.