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Identification of circulating CD90 CD73 cells in cirrhosis of liver

Mitnala Sasikala1, Pugazhelthi Surya, Gaddipati Radhika

  • 1Mitnala Sasikala, Pugazhelthi Surya, Gaddipati Radhika, Pondugala Pavan Kumar, Mekala Subba Rao, Rathindra Mohan Mukherjee, Institute of Basic Sciences and Translational Research, Asian Health Care Foundation, Hyderabad 500082, India.

World Journal of Stem Cells
|August 24, 2011
PubMed

Insights

Circulating CD90(+) CD73(+) CD45(-) cells are more prevalent in cirrhosis patients and exhibit enhanced in vitro proliferation, suggesting a role in liver disease progression.

Area of Science:

  • Cell biology
  • Immunology
  • Hepatology

Background:

  • Liver cirrhosis is a severe condition with limited treatment options.
  • Understanding cellular mechanisms in cirrhosis is crucial for developing new therapies.

Purpose of the Study:

  • To identify and characterize circulating CD90(+) CD73(+) CD45(-) cells in liver cirrhosis.
  • To evaluate the in vitro proliferative capacity of these cells.

Main Methods:

  • Peripheral blood mononuclear cells were isolated from cirrhosis patients and healthy controls.
  • Cells were cultured, and fibroblast-like cells were analyzed for CD90, CD73, and CD45 expression using flow cytometry and immunocytochemistry.
  • Colony-forming efficiency (CFE) was assessed to evaluate proliferative potential.

Main Results:

  • Cirrhosis patients exhibited a significantly higher percentage of CD90(+) CD73(+) CD45(-) cells compared to healthy volunteers.
  • These cells demonstrated a tenfold increase in CFE, indicating enhanced proliferation.
  • Flow cytometry and immunocytochemistry confirmed the expression of CD90(+) CD73(+) markers on proliferating cells.

Conclusions:

  • Circulating CD90(+) CD73(+) CD45(-) cells are present in higher numbers in liver cirrhosis patients.
  • These cells possess a significantly elevated capacity for in vitro proliferation.
  • The findings suggest these cells may play a role in the pathophysiology of liver cirrhosis.
Abstract

Keywords:
CD90+ CD73+ CD45- cellsColony forming efficiencyLiver cirrhosisProliferation

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