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Related Experiment Video

Updated: May 30, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
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Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Control of aptamer function using radiofrequency magnetic field.

Kenichi Taira1, Koichi Abe, Takayuki Ishibasi

  • 1Department of Biotechnology, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Tokyo 184-8588, Japan.

Journal of Nucleic Acids
|August 24, 2011
PubMed
Summary

Radiofrequency magnetic fields (RFMF) can control aptamer function by heating gold nanoparticles conjugated to them. This study demonstrates RFMF can regulate the inhibitory activity of a thrombin-binding aptamer, offering a novel remote control method.

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Molecular Biology

Background:

  • Aptamers offer precise control over protein function.
  • Remote control of aptamer activity is a developing area in biotechnology.

Purpose of the Study:

  • To develop a novel system for remote control of aptamer function using radiofrequency magnetic fields (RFMF).
  • To investigate the effect of RFMF-induced local heating on the structure and inhibitory activity of a gold nanoparticle-conjugated thrombin-binding aptamer (TBA).

Main Methods:

  • Conjugation of a 31-mer thrombin-binding aptamer (TBA) to gold nanoparticles.
  • Application of radiofrequency magnetic fields (RFMF) to induce local heating.
  • Assay of TBA inhibitory activity on thrombin, with a complementary DNA strand used to maintain aptamer structure during the assay.

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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Published on: October 6, 2022

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Last Updated: May 30, 2026

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis
08:09

Mapping the Binding Site of an Aptamer on ATP Using MicroScale Thermophoresis

Published on: January 7, 2017

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
03:38

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Published on: October 6, 2022

Main Results:

  • RFMF irradiation led to a decrease in the inhibitory activity of the gold nanoparticle-conjugated TBA.
  • This observed decrease indicates that RFMF can effectively control the structural conformation of the TBA.
  • The study successfully demonstrated RFMF-mediated control over aptamer function.

Conclusions:

  • Radiofrequency magnetic field-induced local heating of gold nanoparticle-aptamer conjugates provides a novel, non-invasive method for controlling aptamer function.
  • This strategy holds promise for developing new ways to regulate aptamer-based therapeutics.