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Protective effect of quercetin against oxidative stress caused by dimethoate in human peripheral blood lymphocytes
Bochra Gargouri1, Riadh Ben Mansour, Fatma Ben Abdallah
1Unité de Biotechnologie et Pathologies, Institut Supérieur de Biotechnologie de Sfax, 3038 Tunisie. bochragargouri@yahoo.fr
Background:
The aim of this study is to investigate the effect of quercetin in alleviating the cytotoxic effects of Dimethoate in human peripheral blood lymphocytes.
Methods:
Lymphocytes were divided into too groups. The first group, lymphocytes were incubated for 4 h at 37°C with different concentrations (0, 40, 60 and 100 mM) of Dimethoate. The second group was preincubated with quercetin for 30 min and followed by Dim incubation for 4 h at 37°C.
Results:
Following in vitro incubation, Dimethoate caused a significant increase in malondialdehyde levels, a significant decrease in thiol levels, as well as a significant increase in superoxide dismutase, and catalase activities in lymphocytes at different concentrations. Quercetin pretreated lymphocytes showed a significant protection against the cytotoxic effects inducted by Dimethoate on the studied parameters.
Conclusion:
In conclusion, antioxidant quercetin could protect against Dimethoate-induced oxidative stress by decreasing lipid peroxidation, protein oxidation and increasing superoxide dismutase and catalase activities in human lymphocytes.