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Resonance light scattering method for the determination of DNA with cationic methacrylate based polymer nanoparticle

Qi-Chao Zou1, Jin-Zhi Zhang, Shi-Gan Chai

  • 1Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineer, Hubei University, Wuhan 430062, PR China. qichaozou@yahoo.com.cn

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|August 25, 2011
PubMed

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...