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Published on: September 10, 2016
Rational design of a cyclin A fluorescent peptide sensor
Elena Pazos1, Miguel Pérez, Hugo Gutiérrez-de-Terán
1Departamento de Química Orgánica y Centro Singular de Investigación en Química Biolóxica e Materiais Moleculares (CIQUS), Unidad Asociada al CSIC, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Abstract:
We report the design and development of a fluorescent sensor specifically designed to target cyclin A, a protein that plays a key role in the regulation of the cell cycle. Computational studies provide a molecular picture that explains the observed emission increase, suggesting that the 4-DMAP fluorophore in the peptide is protected from the bulk solvent when inserted into the hydrophobic binding groove of cyclin A.
