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Related Concept Videos

Structure of Cardiac Muscles01:13

Structure of Cardiac Muscles

Cardiac muscle, or myocardium, is a specialized type of muscle found exclusively in the heart. Its unique structural and functional characteristics enable the heart to perform its vital role of pumping blood throughout the body continuously and rhythmically. The cardiac muscle cells, or cardiomyocytes, possess an endomysium and perimysium but do not have an epimysium.
Compared to skeletal muscles, cardiac muscle cells are small and mostly have a single nucleus. Additionally, they are usually...

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Related Experiment Video

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Visualization of Cell Cycle Variations and Determination of Nucleation in Postnatal Cardiomyocytes
09:41

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Published on: February 24, 2017

Microtopographical assembly of cardiomyocytes.

Anuj A Patel1, Tejal A Desai, Sanjay Kumar

  • 1The UC Berkeley - UCSF Graduate Program in Bioengineering, USA.

Integrative Biology : Quantitative Biosciences From Nano to Macro
|August 25, 2011
PubMed
Summary

Microtopographic scaffolds with "micropegs" guide cardiac cells to form organized, functional tissue. This approach enhances cardiomyocyte assembly and adhesion for improved cardiac tissue engineering.

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cardiovascular Research

Background:

  • Maintaining three-dimensional tissue architecture is crucial for functional myocardial tissue.
  • Topological cues in scaffolds may facilitate the engineering of functional myocardial tissue.

Purpose of the Study:

  • To investigate the use of microtopographic scaffolds to control cardiac tissue assembly.
  • To explore how micropegs influence cardiomyocyte cytoarchitecture and function.

Main Methods:

  • Designed polydimethylsiloxane (PDMS) scaffolds with microtopographic pillars ("micropegs").
  • Cultured HL-1 cardiomyocytes on these scaffolds and assessed cell growth, adhesion, and function.
  • Utilized Western blots to analyze the expression of junctional markers.

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Fabrication of 3D Cardiac Microtissue Arrays using Human iPSC-Derived Cardiomyocytes, Cardiac Fibroblasts, and Endothelial Cells
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Main Results:

  • HL-1 cardiomyocytes grew, formed myofibrillar structures, and beat spontaneously on micropeg scaffolds.
  • Micropegs promoted a three-dimensional cytoarchitecture on a two-dimensional surface.
  • Specific micropeg arrangements upregulated N-cadherin and connexin 43 expression, enhancing cardiomyocyte function.

Conclusions:

  • Microtopography can provide three-dimensional adhesion for cardiomyocytes.
  • Micropegs offer a method to control the assembly of functional cardiac tissue on 2D surfaces.
  • This technique holds promise for advancing cardiac tissue engineering strategies.