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Related Experiment Video

Updated: May 30, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Enabling technologies for yeast proteome analysis.

Johanna Rees1, Kathryn Lilley

  • 1Cambridge Centre for Proteomics, Cambridge Systems Biology Centre, University of Cambridge, Cambridge, UK. jsr31@cam.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2011
PubMed
Summary

Understanding the yeast proteome remains incomplete, despite advanced genome studies. This review details methods for characterizing yeast proteins and suggests future approaches to identify the remaining 20% and understand protein dynamics.

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Area of Science:

  • Proteomics
  • Yeast Biology
  • Molecular Biology

Background:

  • Yeast genome and transcriptome studies are advanced, but proteome characterization is incomplete.
  • RNA levels do not reliably predict protein abundance or function.
  • Key aspects like post-translational modifications, protein turnover, and interactions require further investigation.

Purpose of the Study:

  • To review current methods for cataloging and characterizing the yeast proteome.
  • To identify future strategies for completing the characterization of the yeast proteome.
  • To gain new insights into protein dynamics within yeast cells.

Main Methods:

  • Review of global and targeted proteomic analysis techniques.
  • Discussion of methods for identifying and characterizing proteins.

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Last Updated: May 30, 2026

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions
08:07

Probing High-density Functional Protein Microarrays to Detect Protein-protein Interactions

Published on: August 2, 2015

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins
10:54

Enzymatic Modification and Flow Cytometry Assessment of Yeast Surface Displayed Proteins

Published on: May 30, 2025

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
09:14

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Published on: June 28, 2018

  • Exploration of approaches to study protein dynamics, including localization and interactions.

    • Main Results:

    • Current methods have advanced yeast proteome characterization but significant gaps remain.
    • Approximately 20% of the yeast proteome is yet to be fully identified and characterized.
    • Existing techniques offer limited insight into protein diversity and post-translational modifications.

    Conclusions:

    • Further proteomic research is essential to fully understand yeast protein landscape.
    • New methodologies are needed to address the remaining 20% of the yeast proteome.
    • Investigating protein dynamics is crucial for a comprehensive understanding of yeast cell function.