Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 30, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Competition experiments coupled with high-throughput analyses for functional genomics studies in yeast.

Daniela Delneri1

  • 1Faculty of Life Sciences, The University of Manchester, Manchester, UK. D.Delneri@manchester.ac.uk

Methods in Molecular Biology (Clifton, N.J.)
|August 25, 2011
PubMed
Summary

High-throughput competition experiments in Saccharomyces cerevisiae reveal gene fitness. Haplo-insufficient genes are broadly important, while haplo-proficient genes show environment-specific roles, particularly under nitrogen limitation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

EASY-C: Extraction and Analysis of Small Yeast Chromosomes-A rapid and universal platform for recovering artificial mini-chromosomes from synthetic Sc2.0 yeast and large plasmids from <i>Saccharomyces cerevisiae</i> and nonconventional yeast species.

Synthetic biology (Oxford, England)·2026
Same author

Genome-wide discovery and phenotyping of non-coding transcripts in A. fumigatus reveals lncRNAs with a role in antifungal drug sensitivity.

Nature communications·2026
Same author

Construction of a reference genome for Starmerella batistae and annotation of Starmerella species reveal an unexpected evolutionary relationship with Schizosaccharomyces pombe and suggest an alternative enzymatic route for sophorolipid production.

FEMS yeast research·2026
Same author

Advances and challenges in understanding evolution through genome comparison: meeting report of the European Molecular Biology Organization (EMBO) lecture course "Evolutionary and Comparative Genomics".

Bioinformatics advances·2025
Same author

Functional analysis and transcriptional profiling of non-coding RNAs in yeast.

The Biochemical journal·2025
Same author

Unveiling the genetic basis of the low pH response in the acidophilic yeast Maudiozyma bulderi as a potential host for biorefinery.

FEMS yeast research·2025

Area of Science:

  • Yeast genetics and genomics
  • Systems biology
  • High-throughput screening

Background:

  • Competition experiments measure yeast strain fitness.
  • The Saccharomyces cerevisiae yeast knock-out (YKO) collection enables simultaneous fitness data retrieval for thousands of genes.
  • Molecular barcodes identify yeast mutants in microarray analyses.

Purpose of the Study:

  • To present a method for high-throughput competition experiments in yeast.
  • To identify genes with haplo-insufficient or haplo-proficient growth phenotypes.
  • To analyze gene fitness across different nutrient-limiting conditions.

Main Methods:

  • Utilizing the Saccharomyces cerevisiae yeast knock-out (YKO) deletion collection.
  • Performing competition experiments in continuous culture with chemically defined media.

More Related Videos

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
09:14

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Published on: June 28, 2018

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
07:55

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

Related Experiment Videos

Last Updated: May 30, 2026

Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens
09:14

Informatic Analysis of Sequence Data from Batch Yeast 2-Hybrid Screens

Published on: June 28, 2018

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing
07:55

High Throughput Yeast Strain Phenotyping with Droplet-Based RNA Sequencing

Published on: May 21, 2020

  • Employing microarray analysis to detect molecular barcodes for strain identification.

    • Main Results:

    • Identified numerous genes exhibiting haplo-insufficient or haplo-proficient phenotypes.
    • Haplo-insufficient genes showed conserved roles across various media.
    • Haplo-proficient genes displayed environment-specific fitness, notably proteasome genes under nitrogen limitation.

    Conclusions:

    • Competition experiments provide effective fitness measurements for yeast strains.
    • Gene essentiality and environmental adaptability can be elucidated through this high-throughput approach.
    • The method allows for detailed discrimination of yeast strain fitness profiles in specific environments.