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Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model
Published on: December 20, 2018
Bdellovibrios, potential biocontrol bacteria against pathogenic Aeromonas hydrophila
Haipeng Cao1, Shan He, Huicong Wang
1Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources of Ministry of Education, National Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai 201306, PR China.
Abstract:
Recent studies have revealed that the use of bdellovibrios is an alternative to control bacteriosis. However, no bdellovibrios are available against Aeromonas hydrophila infections in sturgeons. In the present study, a potential Bdellovibrio strain F16 was isolated from sturgeon gut samples, using a sturgeon-pathogenic A. hydrophila as the prey bacterium. It was initially identified as a Bdellovibrio strain using morphological characteristics and specific PCR amplification, and confirmed to be Bdellovibrio sp. strain ETB (GenBank Accession No. DQ302728) and Bdellovibrio bacteriovorus strain SRA9 (GenBank Accession No. AF263833) by phylogenetic analysis. In addition, it was shown to be safe for mammalians and sturgeons, had a wide prey range, and exhibited significant bacteriolytic effects on the pathogenic A. hydrophila. To the best of our knowledge, this is the first report on a promising gut Bdellovibrio strain against pathogenic A. hydrophila.
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