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The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
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Rapid BAC selection for tol2-mediated transgenesis in zebrafish

Jeroen Bussmann1, Stefan Schulte-Merker

  • 1Hubrecht Institute - KNAW and Utrecht Medical Center, Uppsalalaan 8, 3584 CT, Utrecht, The Netherlands.

Development (Cambridge, England)
|August 26, 2011
PubMed
Summary

This study presents a streamlined protocol for generating zebrafish transgenic lines using modified bacterial artificial chromosomes (BACs). This method enhances the reliability and efficiency of creating stable transgenic zebrafish for research.

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