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Updated: May 29, 2026

Isolation of Enteric Glial Cells from the Submucosa and Lamina Propria of the Adult Mouse
Published on: August 15, 2018
Behind an enteric neuron there may lie a glial cell.
1Department of Pathology and Cell Biology, Columbia University, College of Physicians and Surgeons, 630 West 168th Street, New York, New York 10128, USA. mdg4@columbia.edu
Enteric glia, once thought to be mere support cells in the enteric nervous system (ENS), can act as neuronal precursors. Research shows they can generate new neurons in vitro and in vivo, opening future therapeutic avenues.
Area of Science:
- Neuroscience
- Gastroenterology
- Cell Biology
Background:
- Enteric glia traditionally serve supportive roles in the enteric nervous system (ENS).
- The ENS regulates gastrointestinal function.
- The potential of enteric glia as neuronal precursors was previously unexplored.
Purpose of the Study:
- To investigate whether enteric glia can function as neuronal precursors.
- To determine if neurogenesis can be induced in the adult mouse ENS.
- To explore the potential of enteric glia in ENS repair.
Main Methods:
- In vitro studies assessing enteric glia differentiation into neurons.
- In vivo experiments to induce neurogenesis in the adult mouse ENS.
- Analysis of neuronal replacement and glial plasticity within the ENS.
Main Results:
- Enteric glia were demonstrated to generate neurons in vitro.
- Experimental induction of neurogenesis in the adult mouse ENS was successful.
- Glia do not constitutively replace neurons, and induced neurogenesis is challenging.
Conclusions:
- Enteric glia possess neuronal precursor potential.
- While promising, clinical applications for glial-based neuronal replacement are currently premature.
- These findings open new avenues for ENS research and potential regenerative strategies.
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