[The effects of prolonged sevoflurane anesthesia on renal function in liver fibrosis rabbits]

To determine the effects of prolonged sevoflurane anesthesia on renal function in liver fibrosis rabbits.

Fifty male new zealand rabbits were randomly divided into two groups: fibrosis group (n = 40) and normal control group (Group N, n = 10). In the fibrosis group, experimental liver fibrosis was induced by subcutaneous injection of carbon tetrachloride (CCl4 soluted in oil with the concentration of 500 mL/L). Twelve weeks later, 24 rabbits survived in fibrosis group were randomly allocated to control group (Group F, n = 12) or treating with sevoflurane (Group S, n = 12). Both group S and group N received 1.0 minimum alveolar concentration (MAC) sevoflurane for 8 h with a fresh gas flow rate of 1 L/min. Group F was just given fluid infusion. After 8 h of 1.0 MAC anesthesia, sevoflurane was discontinued. Blood samples were collected before (T0) and 8 h (T8), 24 h (T24), 72 h (T72), and 120 h (T120) after anesthesia. The renal function was assessed by blood urea nitrogen (BUN) and creatinine (Cr). The serum inorganic fluoride was measured by ion-selective electrode. The pathological consequences of the livers were obtained after 5 days or when the rabbits died.

All the rabbits in group S and group F developed liver fibrosis. The hepatic function was significantly abnormal in group S and group F. Aminotransferase (AST), glutamic-pyruvic transaminase (ALT), lactate dehydrogenase (LDH) and total bile acid (TBA) were significantly higher in the fibrosis group (group S and group F) than those in group N. Alkaline phosphatase (ALP) in the fibrosis group was lower than that in group N. There were no significant differences of hepatic function between group S and group F. There were no significant differences in renal function and serum inorganic fluoride before and after anesthesia among the three groups. In group N and S, BUN and serum inorganic fluoride increased slightly at T8, and serum Cr was highest at T72. All of them returned to basic line at T120. In group F, BUN increased slightly at T8, and serum Cr was highest at T72. Serum inorganic fluoride was highest at T120. BUN and serum Cr returned to basic line at T120.

Prolonged sevoflurane anesthesia has no significant influences on renal function in normal and liver fibrosis rabbits.