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Updated: May 29, 2026
Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing
Published on: December 23, 2022
[Population characterization of Balantidium coli from pigs using ITS1-5.8S rRNA-ITS2 sequence]
Tianqi Wang1, Gang Dou, Wenchao Yan
1College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China. wtq@mail.haust.edu.cn
Objective:
To determine the species and genotypes of Balantidium isolated from pigs in Henan province, China.
Methods:
Scatoscopy and the modified DMEM media were used to isolate trophozoites of Balantidium from pig feces. The ITS1-5.8S rRNA-ITS2-based molecular marker method, Acridine orange staining ( AO) and microscopic observation were used to determine the population characteristics among different isolates of B. coli from various pigs farms.
Results:
We isolated 15 isolates from the pigs at diagnosis in the Animal Hospital of Henan University of Science and Technology from the pig farms of the 8 counties or cities of the west of Henan province in total, and all of them belong to the same species B. coli. MJ-2 and SX-1 isolates were genotype A of B. coli, and the remaining 13 isolates were genotype B. Trophozoites of MJ-2 and SX-1 were bigger, moved more slowly and lower density in feces and in vitro culture than other 13 isolates, while structures of their nuclei were not different.
Conclusion:
Both genotype A and B of B. coli are present in the pig farms of the west of Henan province, China, and genotype B is the determinant population in pigs farms. These findings could provide an important implication for the effective control of balantidiosis of human and other hosts.
