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Fully Automated Centrifugal Microfluidic Device for Ultrasensitive Protein Detection from Whole Blood
Published on: April 16, 2016
Hierarchically structured one-dimensional TiO2 for protein immobilization, direct electrochemistry, and mediator-free
Peng Si1, Shujiang Ding, Jun Yuan
1School of Chemical and Biomedical Engineering, Nanyang Technological University, 70 Nanyang Drive, Singapore 637457.
Abstract:
A novel one-dimensional hierarchically structured TiO(2) (1DHS TiO(2)) was synthesized by a solvothermal method using multiwalled carbon nanotubes (MWCNTs) as a template and evaluated for the immobilization of protein and biosensing applications. Characterization studies showed that the 1DHS TiO(2) possessed an anatase crystalline structure and a large surface area with narrow pore size distribution. Fast direct electron transfer was observed for glucose oxidase (GOx) immobilized on the 1DHS TiO(2), and excellent electrocatalytic performance for glucose detection can be obtained without a mediator. The glucose sensor based on the GOx/1DHS TiO(2)-modified electrode had a high sensitivity of 9.90 μA mM(-1) cm(-2) and a low detection limit of 1.29 μM. The fabricated biosensor displayed good selectivity and long-term stability, indicating that the novel structured TiO(2) is a promising material for the immobilization of biomolecules and the fabrication of third-generation biosensors.
