Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mechanism of Conjugation

Mechanism of Conjugation

Bacterial conjugation is a mechanism of horizontal gene transfer that enables the exchange of genetic material between bacterial cells through direct contact. This process is facilitated by a donor cell carrying a conjugative plasmid, which encodes genes necessary for pilus formation, DNA replication, and transfer. The conjugative plasmid plays a central role in initiating and executing the transfer of genetic material.The tra region of the conjugative plasmid encodes proteins responsible for...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Polymer-Grafted Nanoparticles as All-in-One Nanoplatforms.

ACS polymers Au·2026

Same author

Oxygen-Tolerant Photo-Induced Miniaturized Accelerated Atom Transfer Radical Polymerization (OPTIMA-ATRP) for High-Throughput Synthesis of Polymer Bioconjugates.

Angewandte Chemie (International ed. in English)·2026

Same author

Enhanced B-N coordinated dynamic boronate chemistry for recyclable thermosets with elevated stability.

Nature communications·2026

Same author

Processing-Driven Control of the Properties of Polymer Grafted Nanoparticle Composites.

ACS nano·2026

Same author

Neutron Reflectometry Reveals Diffusion in Contrast-Matched Brush Particle Bilayers.

ACS macro letters·2026

Same author

Controlled Synthesis of Liquid-Crystalline Polymers Under Ambient Conditions by Red-Light-Driven ATRP.

Macromolecules·2026

Search research articles

Related Experiment Video

Updated: May 29, 2026

Synthetic Condensates and Cell-Like Architectures from Amphiphilic DNA Nanostructures

Synthetic Condensates and Cell-Like Architectures from Amphiphilic DNA Nanostructures

Published on: May 31, 2024

Direct DNA conjugation to star polymers for controlled reversible assemblies.

Saadyah Averick¹, Eduardo Paredes, Wenwen Li

¹Department of Chemistry and Center for Nucleic Acids Science and Technology, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, United States.

Bioconjugate Chemistry

|August 27, 2011

Summary

Researchers created star-polymer DNA nanoparticles using a click reaction. These customizable nanomaterials can self-assemble and be controllably disassembled, offering new possibilities for nanotechnology applications.

More Related Videos

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

Related Experiment Videos

Last Updated: May 29, 2026

Synthetic Condensates and Cell-Like Architectures from Amphiphilic DNA Nanostructures

Synthetic Condensates and Cell-Like Architectures from Amphiphilic DNA Nanostructures

Published on: May 31, 2024

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation

Published on: December 29, 2021

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

Area of Science:

Biomaterials Science
Nanotechnology
Polymer Chemistry

Background:

Polymer biomolecule hybrids are versatile nanomaterials with tunable properties.
Click chemistry offers efficient and specific conjugation methods for creating complex architectures.

Purpose of the Study:

To synthesize star-polymer DNA nanoparticles using a ligandless click reaction.
To investigate the self-assembly and disassembly capabilities of these novel nanostructures.

Main Methods:

Utilized copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) click reaction for conjugation.
Employed a star-polymer architecture for multivalency and controlled DNA orientation.
Studied nanoassembly formation through DNA hybridization and controlled disassembly.

Main Results:

Successfully synthesized star-polymer DNA conjugates with controlled DNA orientation.
Demonstrated the ability of star-polymer DNA nanoparticles to form higher-order nanoassemblies.
Showcased DNA-mediated controlled disassembly of the nanostructures.

Conclusions:

Star-polymer DNA nanoparticles are a highly customizable and versatile class of nanomaterials.
The developed click chemistry approach enables precise control over nanoparticle architecture and function.
These nanomaterials hold promise for applications in self-assembly and controlled disassembly systems.