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Published on: March 30, 2018
Aberrant antigenic expression in extranodal NK/T-cell lymphoma: a multi-parameter study from Thailand
Tawatchai Pongpruttipan1, Tanawan Kummalue, Anan Bedavanija
1Department of Pathology, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand. watpptp@yahoo.com
Diagnostic Pathology
|August 27, 2011
Summary
Extranodal NK/T-cell lymphoma, nasal type (ENKTL) is characterized by specific immunophenotypic profiles and frequent EBV subtype A with LMP-1 gene deletion. Poor survival was linked to a lack of perforin expression in ENKTL cases.
Area of Science:
- Hematology
- Oncology
- Immunology
Background:
- Extranodal NK/T-cell lymphoma, nasal type (ENKTL) is prevalent in Asia, yet its immunophenotypic profiles are not well-defined.
- Limited series have described ENKTL, necessitating further characterization.
- This study aimed to define ENKTL immunophenotypic profiles and EBV characteristics.
Purpose of the Study:
- To characterize the immunophenotypic profiles of ENKTL.
- To determine the distribution of Epstein-Barr virus (EBV) subtypes in ENKTL.
- To investigate the frequency of LMP-1 gene deletion in ENKTL.
Main Methods:
- Tissue microarray (TMA) and immunohistochemistry were employed.
- Epstein-Barr virus encoded RNA (EBER) in situ hybridization was performed.
- T-cell receptor (TCR) gene rearrangement, EBV subtyping, and LMP-1 gene deletion were analyzed.
Main Results:
- ENKTL cases showed high positivity for TIA-1 (100%), granzyme B (95%), perforin (86%), and CD56 (82%).
- TCR gene rearrangement was negative in all tested cases; EBV subtype A was universal, with 87% showing LMP-1 deletion.
- Lack of perforin expression correlated with significantly poorer survival (p = 0.008).
Conclusions:
- TIA-1 and EBER are highly sensitive markers for ENKTL diagnosis.
- CD3/CD56 negativity and CD45/CD7 loss can occur in ENKTL.
- PCR-based TCR gene rearrangement analysis may not be a reliable diagnostic tool for ENKTL.
