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Related Experiment Video

Updated: May 29, 2026

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

Single-channel Analysis and Calcium Imaging in the Podocytes of the Freshly Isolated Glomeruli

Published on: June 27, 2015

VEGF regulates TRPC6 channels in podocytes.

Florian Thilo¹, Ying Liu, Christoph Loddenkemper

¹Medizinische Klinik, Nephrologie, Charité Campus Benjamin Franklin, Berlin, Germany.

Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association

|August 27, 2011

Summary

Vascular endothelial growth factor (VEGF) increases transient receptor potential canonical type 6 (TRPC6) channels in kidney podocytes. This finding suggests VEGF plays a role in proteinuric kidney diseases by regulating TRPC6 expression and function.

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Last Updated: May 29, 2026

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Area of Science:

Nephrology
Molecular Biology
Cell Biology

Background:

Increased plasma vascular endothelial growth factor (VEGF) and podocyte transient receptor potential canonical type 6 (TRPC6) channels are linked to proteinuric kidney diseases.
The precise relationship between VEGF and TRPC6 in podocytes remained unclear.

Purpose of the Study:

To investigate the hypothesis that VEGF regulates TRPC6 expression and function in podocytes.
To explore the potential role of VEGF-TRPC6 interaction in the pathogenesis of proteinuric kidney diseases.

Main Methods:

Quantitative real-time PCR and immunoblotting to measure TRPC6 mRNA and protein levels in cultured podocytes stimulated with VEGF165.
Confocal microscopy to visualize YFP-tagged TRPC6 in podocytes.
Fluorometric measurement of TRPC6-associated calcium influx.
Immunofluorescence and immunohistochemistry on renal tissue from diabetic nephropathy patients and controls.

Main Results:

VEGF165 administration significantly increased TRPC6 mRNA and protein expression in podocytes, in a dose-dependent manner.
VEGF165 enhanced TRPC6-mediated calcium influx, dependent on de novo protein synthesis and phosphoinositide-3-kinase signaling.
Elevated TRPC6 and VEGF receptor type 2 (VEGFR-2) protein levels were observed in podocytes from patients with diabetic nephropathy.
A significant positive association was found between VEGFR-2 mRNA and TRPC6 mRNA in human renal cortex.

Conclusions:

VEGF directly regulates TRPC6 expression and function in podocytes.
VEGF-induced TRPC6 upregulation may contribute to the pathophysiology of proteinuric kidney diseases, including diabetic nephropathy.