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Mouse- and Human-derived Primary Gastric Epithelial Monolayer Culture for the Study of Regeneration
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Sequential protooncogene expression during regeneration in rat stomach.

T Ito1, N Hayashi, Y Sasaki

  • 1First Department of Medicine, Osaka University Medical School, Japan.

Gastroenterology
|June 1, 1990
PubMed
Summary

Cellular protooncogenes, including c-myc and c-Ha-ras, are key to stomach regeneration after injury. Their expression in gastric cells correlates with cell proliferation during the healing process.

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Area of Science:

  • Gastroenterology
  • Molecular Biology
  • Cell Biology

Background:

  • Cellular protooncogenes like c-myc and c-Ha-ras are implicated in cellular growth and differentiation.
  • Understanding their role in tissue regeneration is crucial for regenerative medicine and disease treatment.

Purpose of the Study:

  • To investigate the expression patterns of c-myc and c-Ha-ras protooncogenes and their oncoproteins during gastric regeneration.
  • To correlate protooncogene expression with cell proliferation in damaged gastric mucosa.

Main Methods:

  • In situ hybridization histochemistry and immunohistochemistry were employed to detect gene and protein expression.
  • 5-bromo-2'-deoxyuridine incorporation was used to identify cells in the S-phase (DNA synthesis).

Main Results:

  • c-myc gene expression peaked at 3 hours, localized to nuclei.
  • c-Ha-ras gene expression peaked at 6-12 hours, localized to cytoplasm.
  • Protooncogene expression was observed in mucous neck, parietal, chief, and enterochromaffin-like cells around injury sites.
  • Cell proliferation (S-phase) distribution aligned with areas of protooncogene expression.

Conclusions:

  • Various gastric mucosal cell types exhibit sequential and regulated expression of cellular protooncogenes during stomach regeneration.
  • These findings highlight the involvement of c-myc and c-Ha-ras in the coordinated cellular response to gastric injury.