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Subunits shared by eukaryotic nuclear RNA polymerases.

N A Woychik1, S M Liao, P A Kolodziej

  • 1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.

Genes & Development
|March 1, 1990
PubMed
Summary
This summary is machine-generated.

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Common subunits of RNA polymerases I, II, and III are encoded by essential, single-copy genes in yeast. These conserved subunits are crucial for shared functions, not catalysis.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genetics

Background:

  • RNA polymerases I, II, and III are essential nuclear enzymes involved in transcribing different RNA types.
  • These polymerases share three common subunits that are immunologically and biochemically indistinguishable.

Purpose of the Study:

  • To isolate and characterize the Saccharomyces cerevisiae genes encoding the common subunits RPB5, RPB6, and RPB8.
  • To investigate the conservation and function of these shared subunits across eukaryotic RNA polymerases.

Main Methods:

  • Gene isolation and sequencing of RPB5, RPB6, and RPB8 in yeast.
  • Deduction of transcriptional start sites for these genes.
  • Immunoprecipitation assays to identify coprecipitated proteins.

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Main Results:

  • RPB5, RPB6, and RPB8 genes are single-copy, essential, and located on different chromosomes.
  • RPB6 immunoprecipitation confirmed its association with subunits of all three RNA polymerases.
  • Sequence similarity of RPB5 with a human homolog indicated evolutionary conservation of common subunits.

Conclusions:

  • The common subunits of nuclear RNA polymerases are highly conserved in eukaryotes.
  • These essential subunits likely play regulatory or structural roles, such as in transcriptional efficiency or enzyme stability, rather than a direct catalytic function.