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Related Concept Videos

G-protein Coupled Receptors01:21

G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
G Protein-coupled Receptors01:15

G Protein-coupled Receptors

G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...

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Related Experiment Video

Updated: May 29, 2026

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
09:40

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum

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Visualizing G protein-coupled receptor signalsomes using confocal immunofluorescence microscopy.

Sudha K Shenoy1

  • 1Department of Medicine, Duke University Medical Center, Durham, NC, USA. sheno002@mc.duke.edu

Methods in Molecular Biology (Clifton, N.J.)
|August 27, 2011
PubMed
Summary

This study visualizes how activated extracellular signal-regulated kinases 1 and 2 (ERK1/2) move within cells. This movement is triggered by the angiotensin II type 1a receptor and mediated by beta-arrestin, revealing new insights into G protein-coupled receptor signaling.

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Last Updated: May 29, 2026

Imaging G-protein Coupled Receptor (GPCR)-mediated Signaling Events that Control Chemotaxis of Dictyostelium Discoideum
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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Biochemistry

Background:

  • G protein-coupled receptors (GPCRs) are key mediators of cellular responses to external stimuli.
  • GPCRs activate signaling kinases, influencing diverse cellular functions.
  • The complex of GPCRs with β-arrestin is crucial for kinase recruitment and activation.

Purpose of the Study:

  • To describe a method for visualizing subcellular distribution changes of activated kinases.
  • To investigate the role of the angiotensin II type 1a receptor in kinase signaling.
  • To understand the spatial dynamics of extracellular signal-regulated kinases 1 and 2 (ERK1/2) activation.

Main Methods:

  • Development of a protocol to visualize activated ERK1/2.
  • Utilizing the angiotensin II type 1a receptor as a model GPCR.
  • Employing techniques to track kinase translocation within cellular compartments.

Main Results:

  • Demonstration of a protocol to observe kinase redistribution.
  • Visualization of agonist-induced changes in ERK1/2 localization.
  • Highlighting the role of GPCRs and β-arrestin in directing kinase activity.

Conclusions:

  • The study provides a method to study GPCR-mediated kinase signaling dynamics.
  • Visualizing kinase translocation offers insights into cellular signal processing.
  • Understanding these mechanisms is vital for deciphering cellular responses to external cues.